食品科学 ›› 2019, Vol. 40 ›› Issue (22): 331-338.doi: 10.7506/spkx1002-6630-20180823-246

• 安全检测 • 上一篇    下一篇

金磁微粒模拟酶检测食品中的葡萄糖

韩博林,关桦楠,龚德状,遇世友,刘晓飞,张娜   

  1. (哈尔滨商业大学食品工程学院,黑龙江 哈尔滨 150076)
  • 出版日期:2019-11-25 发布日期:2019-12-02
  • 基金资助:
    国家自然科学基金面上项目(31370649);国家自然科学基金青年科学基金项目(31201376); 黑龙江省自然科学基金项目(C2016034); 黑龙江省普通本科高等学校青年创新人新才培养计划项目(UNPYSCT-2016060); 中国博士后基金资助项目(2014T70304;2013M531009);黑龙江省博士后资助项目(LBH-Z13002); 哈尔滨商业大学科研项目(17XN026); 哈尔滨商业大学研究生创新科研资金项目(YJSCX2017-462HSD;YJSCX2018-538HSD)

Detection of Glucose in Food Using Gold Magnetic Nanoparticles with Peroxidase Mimicking Activity

HAN Bolin, GUAN Huanan, GONG Dezhuang, YU Shiyou, LIU Xiaofei, ZHANG Na   

  1. (College of Food Engineering, Harbin University of Commerce, Harbin 150076, China)
  • Online:2019-11-25 Published:2019-12-02

摘要: 采用水热法制备Fe3O4纳米粒子,并通过对其表面氨基化与金纳米粒子自组装方法构建金磁微粒(Fe3O4@Au),并表征其性能。在其具有模拟过氧化物酶活性的基础上结合葡萄糖在葡萄糖氧化酶作用下产生H2O2,并与过氧化物酶底物二胺盐产生显色反应的原理,建立可视化检测葡萄糖含量的简便方法,并优化葡萄糖检测体系,并对其选择性和回收率进行分析。结果表明,氨基化的Fe3O4纳米粒子可以有效固载金纳米粒子,Fe3O4@Au饱和磁化强度为43 emu/g。检测体系最优工艺组合为:Fe3O4@Au混悬液质量浓度0.15 g/mL、温度70 ℃、时间50 min。在优化条件下,葡萄糖在1~20 mmol/L范围内具有良好的线性关系,线性相关系数R2为0.992 5,检出限为2.45 μmol/L,加标回收率在96%~104%之间,并具有良好的选择性和稳定性。本研究将拓宽纳米材料模拟酶在食品检测的应用并为葡萄糖检测方法的改进提供一种新的思路。

关键词: 金磁微粒(Fe3O4@Au), 模拟酶, 葡萄糖, 食品检测

Abstract: In this experiment, gold magnetic nanoparticles (Fe3O4@Au) were formed by the self-assembly of Fe3O4 nanoparticles, prepared using the hydrothermal method, after surface amination. The performance of Fe3O4@Au was characterized. A simple method for visual detection of glucose was established on the basis of its peroxidase mimicking activity combined with the fact that glucose oxidase (GOx) catalyzes glucose oxidation to produce H2O2, which reacts to form a color with 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) as a peroxidase substrate. The glucose detection system was optimized and the selectivity and recovery of the method were determined. The results showed that Fe3O4 functionalized with amino groups could be effectively loaded with gold nanoparticles, and the saturation magnetization of Fe3O4@Au was 43 emu/g. The optimum detection conditions were as follows: amount of Fe3O4@Au suspension 0.15 g/mL, temperature 70 ℃, and time 50 min. Under the optimized conditions, a good linear relationship was observed for glucose concentration within the range of 1–20 mmol/L with a correlation coefficient (R2) of 0.992 5. The limit of detection (LOD) was 2.45 μmol/L, and the recoveries for spiked samples were 96%–104%. The proposed method had good selectivity and stability. This study will broaden the application of nanoparticle enzyme mimics in food detection and provide a new idea for improving glucose detection in foods.

Key words: gold magnetic nanoparticles, enzyme mimic, glucose, food detection

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