食品科学 ›› 2018, Vol. 39 ›› Issue (9): 116-120.doi: 10.7506/spkx1002-6630-201809018

• 营养卫生 • 上一篇    下一篇

乳菇菌素D体外抗肿瘤活性及对细胞周期和凋亡的影响

戎瑞雪,王中傲,李晓婵,韩跃华,张 奇,李志鹏,郭云然,曹志然*,王 蓓*   

  1. 河北大学医学院,河北 保定 071000
  • 出版日期:2018-05-15 发布日期:2018-05-15
  • 基金资助:
    国家自然科学基金面上项目(30671385);河北省卫生厅指令性项目(20120134);国家级大学生创新项目(201610075021)

Anti-Tumor Activity of Mitissimol D and Its Effect on Apoptosis and Cell Cycle in Vitro

RONG Ruixue, WANG Zhongao, LI Xiaochan, HAN Yuehua, ZHANG Qi, LI Zhipeng, GUO Yunran, CAO Zhiran*, WANG Bei*   

  1. Medical College, Hebei University, Baoding 071000, China
  • Online:2018-05-15 Published:2018-05-15

摘要: 目的:研究来源于绒白乳菇菌的新型倍半萜类化合物——乳菇菌素D的抗肿瘤活性及对细胞周期和细胞凋 亡的影响。方法:运用噻唑蓝法检测乳菇菌素D对A549、HCT-8、Bel-7402、SMMC7721、HeLa细胞增殖的抑制作 用并计算半数抑制率(half maximal inhibitory concentration,IC50),并以顺铂作为阳性对照;选择对该化合物敏感 的A549细胞株,观察其作用后细胞的形态学改变、细胞凋亡率和细胞周期的变化。结果:乳菇菌素D能够有效抑制 A549、Bel-7402、SMMC7721和HeLa细胞的增殖,作用72 h后的IC50分别为2.46、19.09、18.21、17.05 μg/mL;乳菇 菌素D可引起A549细胞显著的形态学改变,出现凋亡小体;诱导A549细胞凋亡(100 μg/mL作用72 h 时的凋亡率为 32.14%)、使细胞阻滞于S期。结论:该化合物能够抑制多种组织来源的肿瘤细胞增殖,其中A549细胞最敏感;其 作用机制是通过干扰细胞增殖周期而诱导肿瘤细胞凋亡。本实验为乳菇菌素D的开发提供实验依据。

关键词: 绒白乳菇菌, 乳菇菌素D, 抗肿瘤活性, 细胞凋亡, 细胞周期

Abstract: Objective: To investigate the anti-tumor activity of mitissimol D, a novel sesquiterpene compound extracted from Lactarius vellereus, and its effects on cell cycle and apoptosis. Methods: 3-(4,5)-Dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay was used to determine the inhibitory effect of mitissimol D on the proliferation of A549, HCT-8, Bel-7402, SMMC7721 and HeLa cells and to determine the half maximal inhibition concentration (IC50) in comparison with cisplatin as a positive control. A549 cells sensitive to mitissimol D were selected to observe the morphological changes and the changes in cell apoptosis rate and cell cycle. Results: Mitissimol D could effectively inhibit the proliferation of A549, Bel-7402, SMMC7721 and HeLa cells with IC50 (72 h) of 2.46, 19.09, 18.21 and 17.05 μg/mL, respectively. Mitissimol D could cause significant morphological changes of A549 cells, leading to the formation of apoptosomes and it also could induce apoptosis in A549 cells ( 32.14% apoptosis rate at 100 μg/mL after 72 h) and lead to cell block in the S phase. Conclusion: Mitissimol D can significantly inhibit the proliferation of tumor cells from a variety of tissue sources, of which A549 cells are the most sensitive, by interfering with the cell cycle. These findings provide an experimental basis for the development of mitissimol D as a new sesquiterpene compound.

Key words: Lactarius vellereus, mitissimol D, anti-tumor activity, apoptosis, cell cycle

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