食品科学 ›› 2019, Vol. 40 ›› Issue (21): 94-99.doi: 10.7506/spkx1002-6630-20180903-030

• 基础研究 • 上一篇    下一篇

As(Ⅲ)和As(Ⅴ)在香菇栽培基质和子实体中的迁移规律

唐庆强,曹晓钢,夏林兵,陈锦丽,张凤珍,罗顺玉,王丹红,   

  1. (1.三明海关,福建 三明 365000;2.拉萨海关,西藏 拉萨 850001;3.杭州海关,浙江 杭州 310016;4.福州海关,福建 福州 350001)
  • 收稿日期:2019-12-03 修回日期:2019-12-03 出版日期:2019-11-15 发布日期:2019-12-02

Absorption and Accumulation of As(III) and As(V) in Lentinus edodes Fruit Bodies from Culture Medium

TANG Qingqiang, CAO Xiaogang, XIA Linbing, CHEN Jinli, ZHANG Fengzhen, LUO Shunyu, WANG Danhong,   

  1. (1. Sanming Customs, Sanming 365000, China; 2. Lhasa Customs, Lhasa 850001, China; 3. Hangzhou Customs, Hangzhou 310016, China; 4. Fuzhou Customs, Fuzhou 350001, China)
  • Received:2019-12-03 Revised:2019-12-03 Online:2019-11-15 Published:2019-12-02

摘要: 在两个生长年份里向香菇培养料中添加不同含量的亚砷酸盐(As(III))和砷酸盐(As(V))标准溶液,利用高效液相色谱联用电感耦合等离子体质谱法检测香菇及其培养料中5 种形态砷含量,研究香菇子实体对培养料中高毒无机砷的迁移规律。结果表明,香菇子实体对添加0.3~10 mg/kg As(III)和As(V)培养料中As(III)和As(V)的富集系数范围分别为0.36~0.84和0.82~7.53;添加0.33~0.38 mg/kg As(III)和As(V)培养料中As(III)和As(V)的迁移规律方程分别为y=-1.138 9x2+0.921 6x-0.004和y=-9.024 3x2-4.388 1x+0.838 7(x、y分别为培养料和香菇子实体中的As(III)或As(V)含量)。培养料中As(III)和As(V)含量的增加会导致香菇子实体的减产甚至绝收,其临界值为0.33 mg/kg。培养料中的As(III)含量随着栽培时间的延长逐渐减少,而As(V)含量则略有增加,香菇子实体中的有机砷含量也会随着As(III)和As(V)添加量的增加而提高,这可能和As(III)在香菇生长时转化为As(V)或有机砷有关。

关键词: 香菇, 砷, 形态分析, 迁移规律

Abstract: This study was done to evaluate the absorption and accumulation of highly toxic inorganic arsenic (As) species in the fruit bodies of Lentinus edodes from the culture medium added with different amounts of arsenite (As(III)) or arsenate (As(V)) during two consecutive crop years. High performance liquid chromatography coupled with inductivity coupled plasma mass spectrometry (HPLC-ICP-MS) was used to detect the contents of five As species in Lentinus edodes and the corresponding culture medium. The results showed that the accumulation coefficients of As(III) and As(V), when added at levels of 0.3–10 mg/kg, in Lentinus edodes ranged from 0.36 to 0.84 and from 0.82 to 7.53, respectively, and at addition levels of 0.33–0.38 mg/kg, the accumulation in Lentinus edodes could be fitted to the following equations: y = ?1.138 9x2 + 0.921 6x ? 0.004 for As(III), and y = ?9.024 3x2 ? 4.388 1x + 0. 838 7 for As(V), where x and y were the contents of As(III) or As(V) in the culture medium and the fruit bodies, respectively. The yield of Lentinus edodes was reduced even to reach zero with increasing concentration of As(III) or As(V) in the culture medium. The critical value was 0.33 mg/kg for both As species. The content of As(III) in the culture medium gradually decreased with cultivation time, while the content of As(V) increased slightly. The content of organic arsenic in the fruit bodies of Lentinus edodes increase with the addition of As(III) or As(V), which may be related to the transformation of As(III) into As(V) or organic arsenic during the growth of Lentinus edodes.

Key words: Lentinus edodes, arsenic, speciation analysis, migration patterns

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