食品科学 ›› 2019, Vol. 40 ›› Issue (8): 248-255.doi: 10.7506/spkx1002-6630-20180907-077

• 工艺技术 • 上一篇    下一篇

西兰花副产物中萝卜硫素提取、纯化及鉴定

张锦华,郭 楠,杨 妍,李庆鹏,郭 芹,段玉权,哈益明   

  1. 1.山西大学生命科学学院,山西 太原 030006;2.中国农业科学院农产品加工研究所,北京 100193;3.农业部农产品质量安全收贮运管控重点实验室,北京 100193
  • 出版日期:2019-04-25 发布日期:2019-05-05
  • 基金资助:
    公益性行业(农业)科研专项(20150314);河南省科技计划项目(182106000046)

Extraction, Purification and Identification of Sulforaphane from Broccoli Byproducts

ZHANG Jinhua, GUO Nan, YANG Yan, LI Qingpeng, GUO Qin, DUAN Yuquan, HA Yiming   

  1. 1. College of Life Science, Shanxi University, Taiyuan 030006, China; 2. Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 3. Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture, Beijing 100193, China
  • Online:2019-04-25 Published:2019-05-05

摘要: 以西兰花副产物为原料,研究萝卜硫素酶解浸提工艺,并通过单因素试验和响应面试验优化最佳工艺条件。在最佳条件的基础上进行放大实验,通过半制备高效液相色谱纯化和富集萝卜硫素,并应用超高效液相色谱-质谱联用技术对纯化物进行鉴定。结果表明:萝卜硫素最佳提取条件为酶解pH 5.0、酶解温度35 ℃、酶解时间67 min;在此条件下,萝卜硫素提取量为151.39 μg/g,与预测值(156.33 μg/g)无显著差异;纯化和富集后,得到28.62 mg/200 g萝卜硫素,回收率为94.52%,纯度为80.74%。根据萝卜硫素的二级质谱与碎片离子信息,进一步确定该纯化物为萝卜硫素。

关键词: 西兰花副产物, 萝卜硫素, 半制备高效液相色谱, 超高效液相色谱-质谱联用

Abstract: The enzymatic extraction of sulforaphane from broccoli stems and leaves was optimized by one-factor-at-a-time method and response surface methodology. Scale-up experiments were carried out under optimized conditions, and the crude extract was purified by preparative high performance liquid chromatography (HPLC) and identified by ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). The results showed that the optimal extrac tion conditions were determined as follows: pH 5.0, temperature 35 ℃ and time 67 min. Under these conditions, the maximum yield of sulforaphane of 151.39 μg/g (on a dry matter basis) was obtained, which agreed with the predicted value (156.33 μg/g). The yield of purified sulforaphane was 28.62 mg from 200 g of broccoli powder, with a 94.52% recovery and 80.74% purity. The tandem mass spectrum and fragment ion information confirmed the substance as sulforaphane.

Key words: broccoli byproducts, sulforaphane, preparative high performance liquid chromatography, ultra performance liquid chromatography-mass spectrometry (UPLC-MS)

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