关键词: 肌原纤维蛋白, 高压均质, 溶解性, 稳定性, 非热加工

Abstract: In order to elucidate the effect of high pressure homogenization (HPH) cycles on the structure and physicochemical properties of chicken myofibrillar proteins (MPs) in aqueous solutions, HPH treatment was applied to homogenize MP aqueous solutions at a pressure of 103 MPa for 1–6 cycles. The results showed that the solubility of MPs was improved significantly by HPH treatment (P < 0.05). As the number of of HPH treatment cycles increased, the particle size of MPs in aqueous solutions decreased initially and then increased; the apparent viscosity declined and the flowability rose; both the surface hydrophobicity and reactive sulfhydryl group content increased, followed by a decrease. Circular dichroism showed that different HPH cycles had distinct effects on the secondary structures of MPs. Excessive HPH cycles could lead to the aggregation of MPs in aqueous solutions, thereby affecting its solubility and stability. The solubility and stability of MPs remained quite good after 4 cycles of HPH treatment. Therefore, the aqueous solubility of MPs can be controlled by selecting the appropriate cycles of HPH treatment, which will provide a new idea for its application in food processing.

Key words: myofibrillar proteins, high pressure homogenization, solubility, stability, non-thermal processing