食品科学 ›› 2019, Vol. 40 ›› Issue (22): 134-140.doi: 10.7506/spkx1002-6630-20181013-113

• 生物工程 • 上一篇    下一篇

乳扇中高产胞外多糖酵母菌的筛选及其抗氧化活性

赵英杰,张文平,吴剑梅,程新   

  1. (江西农业大学生物科学与工程学院,江西 南昌 330045)
  • 出版日期:2019-11-25 发布日期:2019-12-02
  • 基金资助:
    2015年度国家星火计划项目(2015GA730012);国家级大学生创新创业训练计划项目(201710410002)

Screening for Yeast from Dairy Fan for Production of Antioxidant Exopolysaccharides

ZHAO Yingjie, ZHANG Wenping, WU Jianmei, CHENG Xin   

  1. (College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang 330045, China)
  • Online:2019-11-25 Published:2019-12-02

摘要: 为获得高产胞外多糖的酵母菌株,以云南地区发酵食品乳扇为原料,采用平板分离结合硫酸-苯酚测定法筛选目标酵母菌,并通过形态学、生理生化及分子生物学方法进行菌株鉴定,最终从乳扇中初步分离筛选出9 株产胞外多糖酵母菌,包括3 株胶红酵母(Rhodotorula mucilaginosa)、1 株白假丝酵母(Candida albicans)、4 株涎沫假丝酵母(Candida zeylanoides)及1 株金黄蝶形担孢酵母(Papiliotrema aurea)。其中筛选得到的金黄蝶形担孢酵母DF-12能较好利用葡萄糖、蔗糖及糖蜜等碳源高效合成胞外多糖,在发酵168 h后胞外多糖产量可达3 510 mg/L,具有较好的工业生产潜力。体外抗氧化能力实验表明DF-12菌株所产胞外多糖有一定清除1,1-二苯基-2-三硝基苯肼自由基和超氧阴离子自由基的能力。本研究为酵母菌胞外多糖在食品、药品领域的应用研究提供了一定的基础。

关键词: 乳扇, 酵母菌, 分离, 筛选, 胞外多糖, 抗氧化

Abstract: Nine yeast strains capable of producing exopolysaccharides as determined by the phenol/sulfuric acid method were isolated and purified from dairy fan, a traditional fermented milk product in Yunan, China by the streak plate method. These strains were identified through morphological, physiological characteristics and molecular biology techniques, including 3 strains of Rhodotorula mucilaginosa, 1 strain of Candida albicans, 4 strains of Candida zeylanoides and 1 strain of Papiliotrema aurea. A high-yield exopolysaccharide (EPS)-producing strain, namely P. aureus DF-12, was obtained, which could synthesize EPS by using various carbon sources such as glucose, sucrose and molasses, and the highest yield of EPS of 3 510 mg/L was obtained after 168 h fermentation at 28 ℃ using sucrose as carbon source. In vitro antioxidant tests indicated that the EPS from DF-12 had strong scavenging ability against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide anion radicals. The experimental results provide a basis for the development and application of EPS in the food and pharmaceutical industries.

Key words: dairy fan, yeast, isolation, screening, exopolysaccharide, antioxidant activity

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