婴儿源动物双歧杆菌消化道胁迫抗性及体外对免疫细胞活性影响的比较

doi:10.7506/spkx1002-6630-20181017-178

食品科学 ›› 2019, Vol. 40 ›› Issue (22): 118-125.doi: 10.7506/spkx1002-6630-20181017-178

婴儿源动物双歧杆菌消化道胁迫抗性及体外对免疫细胞活性影响的比较

万峰，孙思睿，侯雨佳，赵桉，张晟，孟祥晨

（东北农业大学乳品科学教育部重点实验室，黑龙江哈尔滨 150030）

出版日期:2019-11-25 发布日期:2019-12-02
基金资助:
“十三五”国家重点研发计划重点专项（2017YFD0400304）

Comparison of Resistance of Bifidobacterium animalis Strains Isolated from Infants to Simulated Gastrointestinal Stress and Effect of Selected Strain on Activities of Immune Cells in Vitro

WAN Feng, SUN Sirui, HOU Yujia, ZHAO An, ZHANG Sheng, MENG Xiangchen

(Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China)

Online:2019-11-25 Published:2019-12-02

摘要/Abstract

摘要： 以4 株婴儿源动物双歧杆菌乳亚种（Bifidobacterium animalis subsp. lactis）为研究对象，分析菌株对酸、胆盐、模拟消化道环境的耐受能力及对致病菌的黏附抑制作用，并评价体外对Caco-2细胞的黏附性。此外，测定菌株合成胞外多糖的能力，并探讨其与耐受性及黏附性的关联。经上述实验获得黏附性及抗性双优的菌株H15-2，再经脾淋巴细胞增殖实验、巨噬细胞能量代谢水平及巨噬细胞吞噬中性红能力3 个方面评价其免疫调节能力。结果表明：动物双歧杆菌H15-2对模拟胃肠道环境有较强的耐受性，对Caco-2细胞有较高的黏附能力，并对免疫细胞活性具有较好的调节作用，有应用开发潜力。

关键词: 动物双歧杆菌, 耐受, 黏附, 胞外多糖, 免疫调节

Abstract: Four strains of Bifidobacterium animalis subsp. lactis isolated from infants were evaluated for tolerance to acids, bile salts and simulated digestive tract environment, adhesiveness to Caco-2 cells and inhibitory effects on pathogen adhesion. In addition, the ability to synthesize exopolysaccharides was determined and the relationship between tolerance and adhesion was discussed. Strain H15-2, with good environmental tolerance and adhesiveness, was further evaluated for its immunomodulatory activity by considering splenocyte proliferation, energy metabolism level in macrophages and macrophage phagocytosis of neutral red. The results showed that B. animalis H15-2 had strong tolerance to simulated gastrointestinal environment, high adhesion ability to Caco-2 cells, and good regulatory effects on the activities of immune cells. This study indicates that B. animalis H15-2 has great potential for application development.

Key words: Bifidobacterium animalis, tolerance, adhesion, exopolysaccharides, immunoregulation

中图分类号:

TS201.3

万峰，孙思睿，侯雨佳，赵桉，张晟，孟祥晨. 婴儿源动物双歧杆菌消化道胁迫抗性及体外对免疫细胞活性影响的比较[J]. 食品科学, 2019, 40(22): 118-125.

WAN Feng, SUN Sirui, HOU Yujia, ZHAO An, ZHANG Sheng, MENG Xiangchen. Comparison of Resistance of Bifidobacterium animalis Strains Isolated from Infants to Simulated Gastrointestinal Stress and Effect of Selected Strain on Activities of Immune Cells in Vitro[J]. FOOD SCIENCE, 2019, 40(22): 118-125.

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婴儿源动物双歧杆菌消化道胁迫抗性及体外对免疫细胞活性影响的比较

Comparison of Resistance of Bifidobacterium animalis Strains Isolated from Infants to Simulated Gastrointestinal Stress and Effect of Selected Strain on Activities of Immune Cells in Vitro

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