食品科学 ›› 2019, Vol. 40 ›› Issue (24): 167-172.doi: 10.7506/spkx1002-6630-20181029-338

• 生物工程 • 上一篇    下一篇

蓝莓采后主要病原菌的分离鉴定及肉桂精油抑菌效果

王丹,张静,贾晓曼,辛力,翟浩   

  1. (山东省果树研究所,山东 泰安 271000)
  • 出版日期:2019-12-25 发布日期:2019-12-24
  • 基金资助:
    山东省果树研究所青年科研基金项目(2018KY06);“十三五”国家重点研发计划重点专项(2017YFD0401303); 山东省现代农业产业技术体系水果创新团队专项(SDAIT-06-13)

Identification of Dominant Postharvest Pathogens of Blueberry Fruit and Antifungal Activity of Cinnamon Oil against Them

WANG Dan, ZHANG Jing, JIA Xiaoman, XIN Li, ZHAI Hao   

  1. (Shandong Institute of Pomology, Taian 271000, China)
  • Online:2019-12-25 Published:2019-12-24

摘要: 为明确山东泰安地区蓝莓采后主要致腐病原菌及肉桂精油对其抑菌效果,从自然腐烂的“晚丰”蓝莓上分离纯化优势菌株,采用传统真菌形态学鉴定方法结合rDNA-ITS序列分析方法进行鉴定,并采用体外熏蒸抑菌实验研究肉桂精油对几种主要致病菌的抑制效果。结果表明,泰安本地蓝莓分离到3 株病原真菌,分别为尖孢镰刀菌(Fusarium oxysporum)、链格孢菌(Alternaria alternata)和灰葡萄孢菌(Botrytis cinerea)。体外抑菌实验表明,肉桂精油对尖孢镰刀菌、链格孢菌和灰葡萄孢菌均有较好的抑制效果,其最小抑菌浓度分别为60、30 μL/L和30 μL/L;通过扫描电镜可以看出,与对照组相比,经肉桂精油处理的3 种病原菌菌丝形态均受到严重破坏,发生凹陷、皱缩、粗糙等一系列变形现象。

关键词: 蓝莓, 采后病原菌, 分离鉴定, 肉桂精油, 抑菌效果

Abstract: This study aimed to identify the main postharvest pathogens of blueberry fruit grown in Taian, Shandong province. The pathogenic strains were isolated and purified from naturally rotten fruit of the ‘Wanfeng’ cultivar, and they were identified by traditional fungal morphology and rDNA-ITS sequence analysis. Besides, the in vitro antifungal activity of cinnamon oil was assessed against the strains. The results showed that these strains were identified as Fusarium oxysporum, Alternaria alternate and Botrytis cinerea, respectively. Cinnamon oil exhibited obvious antifungal effects on the strains with minimal inhibitory concentrations of 60, 30 and 30 μL/L, respectively. Moreover, scanning electron microscopic observation showed that the mycelial morphology of all the strains was seriously damaged after being treated with cinnamon oil and the cell surface became sunken, shrunk and uneven compared to the untreated control.

Key words: blueberry, postharvest pathogen, isolation and identification, cinnamon oil, antifungal effect

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