食品科学 ›› 2018, Vol. 39 ›› Issue (11): 53-59.doi: 10.7506/spkx1002-6630-201811009

• 基础研究 • 上一篇    下一篇

MgSO4处理对西兰花芽苗菜生理活性物质和抗氧化能力的影响

初 婷,彭 畅,郭丽萍*   

  1. 青岛农业大学食品科学与工程学院,山东 青岛 266109
  • 出版日期:2018-06-15 发布日期:2018-06-06
  • 基金资助:
    山东省自然科学基金青年科学基金项目(ZR2016CQ17);青岛农业大学高层次人才科研基金项目(6631115051);青岛农业大学2016年大学生科技创新项目

Effect of MgSO4 Treatment on Bioactive Compounds and Antioxidant Activity in Broccoli Sprouts

CHU Ting, PENG Chang, GUO Liping*   

  1. College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China
  • Online:2018-06-15 Published:2018-06-06

摘要: 本实验研究了外源喷施0、25、50、75 mmol/L的MgSO4对西兰花芽苗菜生长状况、生理活性物质含量和抗 氧化能力的影响。结果表明:MgSO4处理对西兰花芽苗菜造成了生长胁迫效应;叶绿素和总酚含量随着MgSO4浓度 的增大而增加,抗坏血酸含量却随着MgSO4浓度的增加而降低。发芽5 d的西兰花芽苗菜中总硫代葡萄糖苷(简称 硫苷)和萝卜硫素含量显著高于发芽8 d的,但发芽8 d的黑芥子酶活力高于发芽5 d的;MgSO4处理后,总硫苷和萝 卜硫素含量及黑芥子酶活力显著提高,但50 mmol/L和75 mmol/L MgSO4处理组之间差异不显著。MgSO4处理显著提 高了西兰花芽苗菜的抗氧化能力,但1,1-二苯基-2-三硝基苯肼自由基清除率在50 mmol/L和75 mmol/L处理组之间无 显著差异,H2O2清除率随MgSO4浓度的增加逐渐增强。因此,综合考虑,50 mmol/L MgSO4对于提高西兰花芽苗菜 的营养价值和保健功能是较适合的浓度。

关键词: 西兰花芽苗菜, 硫苷, 异硫氰酸酯, 总酚, 抗坏血酸, 抗氧化能力

Abstract: The growth, bioactive compound contents and antioxidant activity of broccoli sprouts sprayed with exogenous MgSO4 at 0, 25, 50 and 75 mmol/L were studied in this investigation. The results showed that MgSO4 inhibited the growth of broccoli sprouts. The contents of chlorophyll and total phenolics increased with the increase of MgSO4 concentration; however, ascorbic acid content continuously reduced. In addition, 5-day-old sprouts contained more glucosinolates and sulforaphane than 8-day-old sprouts, whereas the opposite was observed for myrosinase activity. MgSO4 treatment significantly enhanced the contents of glucosinolates and sulforaphane as well as myrosinase activity, but no significant difference was found between 50 and 75 mmol/L treatments. Antioxidant ability was significantly promoted by MgSO4 treatment. Specially, no significant difference in 1,1-diphenyl-2-picrylhydrazyl radical scavenging capacity was observed between 50 and 75 mmol/L MgSO4 groups. Nevertheless, H2O2 scavenging capacity was gradually enhanced with the increase of MgSO4 concentration. These results suggest that 50 mmol/L MgSO4 treatment could be useful to improve the nutritional value and health-promoting function of broccoli sprouts.

Key words: broccoli sprouts, glucosinolates, sulforaphane, total phenolics, ascorbic acid, antioxidant ability

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