食品科学 ›› 2019, Vol. 40 ›› Issue (9): 256-261.doi: 10.7506/spkx1002-6630-20181109-098

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L-半胱氨酸处理对采后李果实褐腐菌的抑制作用

令 阳1,邓丽莉1,2,姚世响1,2,曾凯芳1,2,*   

  1. 1.西南大学食品科学学院,重庆 400715;2.西南大学食品贮藏与物流研究中心,重庆 400715
  • 出版日期:2019-05-15 发布日期:2019-05-28
  • 基金资助:
    公益性行业(农业)科研专项(201303075)

Inhibitory Effect of L-Cysteine against Monilinia fructicola on Postharvest Plum Fruit

LING Yang1, DENG Lili1,2, YAO Shixiang1,2, ZENG Kaifang1,2,*   

  1. 1. College of Food Science, Southwest University, Chongqing 400715, China; 2. Food Storage and Logistics Research Center, Southwest University, Chongqing 400715, China
  • Online:2019-05-15 Published:2019-05-28

摘要: 为探究L-半胱氨酸对采后李果实褐腐菌的抑制作用,本实验通过离体实验探究不同质量浓度(1、10、100、1000 mg/L)L-半胱氨酸对李果实褐腐菌Monilinia fructicola的抑制效果及可能的抑菌机制;采用损伤接种的方法评价L-半胱氨酸处理对李果实采后褐腐病的防治效果。结果表明:在所选的4 个L-半胱氨酸处理质量浓度中,只有1 000 mg/L L-半胱氨酸处理与对照相比可以显著延缓褐腐菌孢子的萌发,抑制菌丝体的生长,增加褐腐菌菌丝体胞外电导率和核酸物质的释放量,破坏褐腐菌孢子细胞膜的完整性(P<0.05);且在果实损伤接种实验中,1000 mg/L L-半胱氨酸处理可显著降低李果实的损伤接种发病率和病斑直径(P<0.05),而1、10、100 mg/L L-半胱氨酸处理对采后李果实褐腐病无明显控制效果。

关键词: L-半胱氨酸, 采后, 李果实, 褐腐菌, 抑制作用

Abstract: The objective of this work was to investigate the inhibitory effect of L-cysteine on brown rot caused by Monilinia fructicola on postharvest plum fruit. We explored the in vitro inhibitory effect and possible mechanism of L-cysteine at various concentrations (1, 10, 100 and 1 000 mg/L) on M. fructicola, and we further explored its effect on the control of postharvest brown rot on plum fruit induced by stab inoculation. The results showed that only 1 000 mg/L L-cysteine treatment could significantly delay the spore germination, inhibit the mycelial growth of M. fructicola, increase the membrane permeability and the leakage of intracellular nucleic acid, and destroy the cell membrane integrity (P < 0.05). Moreover, L-cysteine treatment at 1 000 mg/L could reduce disease incidence and lesion diameter in wounded and inoculated plum fruit when compared with the control fruit (P < 0.05), while at the other concentrations it had no significant effectiveness against brown rot on postharvest plum fruit.

Key words: L-cysteine, postharvest, plum fruit, Monilinia fructicola, inhibitory effect

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