采前喷施胺鲜酯对采后龙眼果实贮藏期间果皮活性氧代谢的影响

doi:10.7506/spkx1002-6630-20181121-236

食品科学 ›› 2019, Vol. 40 ›› Issue (19): 242-248.doi: 10.7506/spkx1002-6630-20181121-236

采前喷施胺鲜酯对采后龙眼果实贮藏期间果皮活性氧代谢的影响

林毅雄，林河通,，陈艺晖，王慧，林艺芬,

（1.福建农林大学食品科学学院，福建福州 350002；2.福建农林大学农产品产后技术研究所，福建福州 350002；3.亚热带特色农产品采后生物学（福建农林大学）福建省高等学校重点实验室，福建福州 350002）

出版日期:2019-10-15 发布日期:2019-10-25
基金资助:
国家自然科学基金青年科学基金项目（31701653）；国家自然科学基金面上项目（30972070；311711776）；福建省自然科学基金项目（2015J01608）；福建省财政厅科技项目（KAA16010A；K81MLV01A）

Effect of Preharvest Spraying of Diethyl Aminoethyl Hexanoate on Metabolism of Reactive Oxygen Species in Longan Fruit Pericarp during Postharvest Storage

LIN Yixiong, LIN Hetong,, CHEN Yihui, WANG Hui, LIN Yifen,

(1. College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2. Institute of Postharvest Technology of Agricultural Products, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 3. Key Laboratory of Postharvest Biology of Subtropical Special Agricultural Products (Fujian Agriculture and Forestry University), Fujian Province University, Fuzhou 350002, China)

Online:2019-10-15 Published:2019-10-25

摘要/Abstract

摘要： 为研究采前喷施胺鲜酯对采后龙眼果实贮藏期间果皮活性氧代谢的影响，以‘福眼’龙眼为材料，在龙眼盛花期后70、90、110 d用10 mg/kg胺鲜酯喷施龙眼果实3 次，以蒸馏水喷施为对照，龙眼果实在盛花期后120 d采收，采后龙眼果实经过挑选、清洗和晾干后用0.015 mm厚的聚乙烯薄膜袋密封包装（每袋50 个），在（28±1）℃、相对湿度85%条件下贮藏，贮藏期间每天取样测定果皮O2－·产生速率、丙二醛（malondialdehyde，MDA）含量、活性氧清除酶（超氧化物歧化酶（superoxidase dismutase，SOD）、过氧化氢酶（catalase，CAT）、抗坏血酸过氧化物酶（ascorbate peroxidase，APX）和谷胱甘肽还原酶（glutathione reductase，GR））活力、内源抗氧化物质（还原型抗坏血酸（ascorbic acid，AsA）和还原型谷胱甘肽（glutathione，GSH））含量及1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl，DPPH）自由基清除率和总还原力的变化。结果发现：与对照果实对比，采前喷施胺鲜酯可提高采后龙眼果实贮藏期间果皮SOD、CAT、APX、GR等活性氧清除酶活力，延缓AsA和GSH含量的下降，保持较高DPPH自由基清除率和总还原力，降低O2－·产生速率和膜脂过氧化产物MDA含量。结论：采前喷施胺鲜酯增强采后龙眼果实耐贮性与其能有效降低采后龙眼果实贮藏期间果皮活性氧代谢有关。

关键词: 龙眼果实, 胺鲜酯, 活性氧, 活性氧代谢, 活性氧清除酶, 耐贮性

Abstract: We studied effects of preharvest spraying of diethyl aminoethyl hexanoate (DA-6) on the metabolism of reactive oxygen species (ROS) in longan fruit pericarp during postharvest storage. Fruit of ‘Fuyan’ longan (Dimocarpus longan Lour. cv. Fuyan) were sprayed with 10 mg/kg DA-6 or distilled water (as control) at 70, 90 and 110 days after full bloom. The fruit were harvested at 120 days, cleaned and naturally dried prior to packaging in 0.015 mm thick polyethylene bags (50 fruits per bag) and subsequent storage at (28 ± 1) ℃ and 85% relative humidity. During storage, superoxide anion radical production rate, malondialdehyde (MDA) content, the contents of endogenous antioxidant substances such as ascorbic acid (AsA) and glutathione (GSH), the activity of ROS scavenging enzymes including superoxidase dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and reducing power were determined daily. The results showed that compared with the control fruit, pre-harvest spraying of DA-6 could increase the activities of SOD, CAT, APX and GR, delay the decrease in AsA and GSH contents, maintain higher levels of DPPH radical scavenging activity and reducing power, lower superoxide anion radical production rate, and reduce the content of MDA in longan fruit pericarp, thereby improving fruit storability. This effect may be due to reduced ROS metabolism in the pericarp of harvested longan fruit during storage.

Key words: longan fruit, diethyl aminoethyl hexanoate, reactive oxygen species (ROS), ROS metabolism, ROS scavenging enzymes, storability

中图分类号:

TS255.36

林毅雄，林河通,，陈艺晖，王慧，林艺芬,. 采前喷施胺鲜酯对采后龙眼果实贮藏期间果皮活性氧代谢的影响[J]. 食品科学, 2019, 40(19): 242-248.

LIN Yixiong, LIN Hetong,, CHEN Yihui, WANG Hui, LIN Yifen,. Effect of Preharvest Spraying of Diethyl Aminoethyl Hexanoate on Metabolism of Reactive Oxygen Species in Longan Fruit Pericarp during Postharvest Storage[J]. FOOD SCIENCE, 2019, 40(19): 242-248.

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采前喷施胺鲜酯对采后龙眼果实贮藏期间果皮活性氧代谢的影响

Effect of Preharvest Spraying of Diethyl Aminoethyl Hexanoate on Metabolism of Reactive Oxygen Species in Longan Fruit Pericarp during Postharvest Storage

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