食品科学 ›› 2019, Vol. 40 ›› Issue (22): 299-305.doi: 10.7506/spkx1002-6630-20181128-341

• 工艺技术 • 上一篇    下一篇

蛹虫草新型黄色素结构的初步鉴定及响应面优化提取工艺

唐鸿标,陈楚欣,林俊芳,,娄海伟,叶志伟,郭丽琼,   

  1. (1.华南农业大学食品学院,广东 广州 510642;2.广东省微生态制剂工程技术研究中心,广东 广州 510642)
  • 出版日期:2019-11-25 发布日期:2019-12-02
  • 基金资助:
    国家自然科学基金面上项目(31572178;31772373;31801918);广东省科技项目(2014B020205003;2016A030313404); “十三五”国家重点研发计划重点专项(2017YFD0400204-03);广东省现代农业技术体系创新团队建设项目(2019KJ103)

New Yellow Pigment from Cordyceps militaris: Structural Identification and Optimization of Extraction by Response Surface Methodology

TANG Hongbiao, CHEN Chuxin, LIN Junfang,, LOU Haiwei, YE Zhiwei, GUO Liqiong,   

  1. (1. College of Food Science, South China Agricultural University, Guangzhou 510642, China; 2. Research Center for Micro-Ecological Agent Engineering and Technology of Guangdong Province, Guangzhou 510642, China)
  • Online:2019-11-25 Published:2019-12-02

摘要: 为研究蛹虫草黄色素的种类并建立最佳的提取工艺,利用高效液相色谱、高分辨质谱和傅里叶变换红外光谱对蛹虫草黄色素进行分离纯化及结构鉴定,结果发现一种新型的蛹虫草黄色素,并将其命名为虫草烯。虫草烯是一种与类胡萝卜素具有相似紫外-可见光、红外吸收特征的新型色素。响应面试验结果表明,虫草烯最佳提取工艺条件为乙醇溶液体积分数57.68%、提取温度44.40 ℃、提取时间52.44 min,并最终确定蛹虫草子实体中虫草烯提取量为(2 780.97±170.38)μg/g。

关键词: 蛹虫草, 黄色素, 结构鉴定, 虫草烯, 提取优化, 响应面

Abstract: This study was done in order to investigate the yellow pigment composition of Cordyceps militaris and to determine the optimal processing conditions for the extraction of?yellow pigment from C. militaris. We applied high performance liquid chromatography (HPLC), high-resolution mass spectrometry (HRMS) and Fourier transform infrared (FTIR) spectroscopy to the separation, purification and structural identification of yellow pigment. A new yellow pigment was found and named cordycepene, which had similar UV-visible and infrared absorption characteristics to carotenoids. By using the response surface methodology, the optimal extraction conditions were obtained as follows: 57.68% ethanol as the extraction solvent and extraction for 52.44 min at 44.40 ℃. Under these optimized conditions, the highest yield of cordycepene of (2 780.97 ± 170.38) μg/g was obtained.

Key words: Cordyceps militaris, yellow pigment, structural identification, cordycepene, extraction optimization, response surface methodology

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