食品科学 ›› 2018, Vol. 39 ›› Issue (13): 198-204.doi: 10.7506/spkx1002-6630-201813029

• 营养卫生 • 上一篇    下一篇

硒化低聚氨基多糖对RAW264.7细胞的免疫调节作用

顾丽霞1,郑 斌1,2,相兴伟1,2,*,闻正顺1,*,周宇芳2,马剑茵1,曲有乐1   

  1. 1.浙江海洋大学食品与医药学院,浙江 舟山 316022;2.浙江省海洋开发研究院,浙江 舟山 316021
  • 出版日期:2018-07-15 发布日期:2018-07-09
  • 基金资助:
    浙江省自然科学基金项目(LY17C170001);国家国际科技合作项目(2015DFA30980);国家自然科学基金青年科学基金项目(31301982);舟山市科技计划项目(2016C41015)

Immunomodulatory Effect of Low-Molecular-Mass Seleno-Aminopolysaccharide in RAW264.7 Macrophages

GU Lixia1, ZHENG Bin1,2, XIANG Xingwei1,2,*, WEN Zhengshun1,*, ZHOU Yufang2, MA Jianyin1, QU Youle1   

  1. 1. School of Food Science and Pharmaceutics, Zhejiang Ocean University, Zhoushan 316022, China; 2. Zhejiang Marine Development Research Institute, Zhoushan 316021, China
  • Online:2018-07-15 Published:2018-07-09

摘要: 本实验旨在研究硒化低聚氨基多糖对小鼠巨噬细胞RAW264.7免疫功能的影响。用噻唑蓝比色法分别考 察硒化低聚氨基多糖、Na2SeO3对巨噬细胞RAW264.7增殖能力的影响,并观察巨噬细胞形态的变化。通过中性红 法、酶联免疫吸附测定法及实时荧光定量反转录聚合酶链式反应法分别检测巨噬细胞的吞噬能力、肿瘤坏死因子 (tumor necrosis factor-α,TNF-α)、白介素(interleukin,IL)-6及IL-10细胞因子的分泌量及其mRNA表达水平。 结果表明:硒质量浓度为100~1 000 μg/L时,硒化低聚氨基多糖对细胞的相对增殖率无显著影响;而Na2SeO3的硒 质量浓度超过200 μg/L即对巨噬细胞RAW264.7产生细胞毒性作用。硒化低聚氨基多糖能增强巨噬细胞吞噬活性, 显著提高RAW264.7细胞TNF-α、IL-6及IL-10分泌量及其mRNA表达水平,且效果比Na2SeO3处理组、低聚氨基多糖 处理组及Na2SeO3+低聚氨基多糖复合添加组好。实验结果提示硒化低聚氨基多糖对小鼠巨噬细胞RAW264.7具有一 定的免疫调节作用。

关键词: 硒, 硒化低聚氨基多糖, 巨噬细胞, 免疫调节

Abstract: The aim of the present study was to investigate the immunomodulatory effect of low-molecular-mass selenoaminopolysaccharide (LSA) in RAW264.7 cells. The effects of LSA and sodium selenite (Se) on the proliferation of RAW264.7 cells were detected by MTT assay. Neutral red test was used to investigate the effect of LSA on phagocytosis and cell morphology in RAW264.7 cells. Enzyme-linked immunoabsorbent assay and real-time fluorescent quantitative reverse transcription-polymerase chain reaction were used to explore the effects of different selenium concentrations of LSA on the secretion and mRNA expression of cytokines (tumor necrosis factor-α (TNF-α), interleukin (IL)-6, IL-10) in RAW264.7 cells. The results revealed that LSA did not affect cell viability at selenium concentrations between 100 and 1000 μg/L. Sodium selenite significantly inhibited cell viability at selenium concentrations more than 200 μg/L. LSA contributed significantly to phagocytic activity in RAW264.7 cells. In addition, LSA could significantly increase the secretion and gene expression levels of TNF-α, IL-6 and IL-10 in RAW264.7 cells. Taken together, these findings suggested that LSA has immunomodulatory activity on macrophage cell line RAW264.7.

Key words: selenium, low-molecular-mass seleno-aminopolysaccharide, macrophage, immunomodulatory

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