软枣猕猴桃黄酮对过氧化氢诱导HaCaT细胞损伤的保护作用

doi:10.7506/spkx1002-6630-201813034

食品科学 ›› 2018, Vol. 39 ›› Issue (13): 229-234.doi: 10.7506/spkx1002-6630-201813034

软枣猕猴桃黄酮对过氧化氢诱导HaCaT细胞损伤的保护作用

石浩，王仁才*，吴小燕，刘琼

湖南农业大学园艺园林学院，国家植物功能成分利用工程技术研究中心，湖南长沙 410128

出版日期:2018-07-15 发布日期:2018-07-09
基金资助:
湖南省研究生科研创新项目（CX2017B357）；猕猴桃镉低积累品种筛选及配套技术研究与示范项目（5026301/111502600009）；湖南省教育厅优秀青年科学研究项目（16B188）

Protective Effect of Actinidia arguta Flavonoid on Hydrogen Peroxide-Induced Injury in HaCaT Cells

SHI Hao, WANG Rencai*, WU Xiaoyan, LIU Qiong

National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410128, China

Online:2018-07-15 Published:2018-07-09

摘要/Abstract

摘要： 研究软枣猕猴桃中黄酮类化合物对过氧化氢（H2O2）损伤人永生化表皮细胞（HaCaT细胞）的保护作用。以湖南省浏阳市大围山软枣猕猴桃为实验材料，以体外培养的HaCaT细胞为实验对象。实验分为空白对照组、H2O2 模型组、阳性对照组（VC组）和黄酮处理组（软枣猕猴桃黄酮0.1～1.0 mg/mL预处理），以细胞内超氧化物歧化酶（superoxide dismutase，SOD）活力、活性氧簇（reactive oxygen species，ROS）水平为综合评价指标，并以VC作为阳性对照评价其抗氧化活性。实验结果表明：当H2O2浓度为30 μmol/L时，细胞相对存活率为40%左右，较好地诱导了HaCaT细胞氧化损伤模型。当加入黄酮类化合物对细胞进行预保护后，质量浓度0.3、0.6 mg/mL的黄酮处理组细胞相对存活率为60%左右，较模型组均提高了20%左右。0.3 mg/mL黄酮处理组细胞内SOD活力（6.33 U/mg）较模型组升高了约1 倍，且存在明显差异（P＜0.05）；ROS水平显著下降，与模型组（677.80）相比下降了13%左右。说明软枣猕猴桃中黄酮类化合物对H2O2造成的氧化损伤具有一定的预保护作用，可进一步将其开发成相应的天然抗氧化剂产品投入市场。

关键词: 软枣猕猴桃, 黄酮, 过氧化氢诱导氧化损伤, HaCaT细胞损伤, 保护作用

Abstract: The protective effect of Actinidia arguta flavonoids (AAF) on H2O2-induced oxidative injury in human immortalized epidermal cells (HaCaT) was investigated in this study. The flavonoids were extracted from A. arguta grown in DaWei Mountain, Liuyang, Hunan provice. HaCaT cells were cultured in vitro. The experiment was divided into control, hydrogen peroxide damage, positive control (VC), and AAF treatment groups (at concentrations of 0.1–1.0 mg/mL). Superoxide dismutase (SOD) activity and reactive oxygen species (ROS) levels in cells were measured to evaluate the cytoprotective effect of AAF. VC was used as a positive control to evaluate its antioxidant activity. The results showed that at a H2O2 concentration of 30 μmol/L, the survival rate of the cells was approximately 40% and the oxidative damage model was established. When AAF at concentrations of 0.3 and 0.6 mg/mL was used to protect the cells, the survival rate was about 60%, which increased by about 20% compared with the model group. SOD activity (6.33 U/mg) in the treatment group (0.3 mg/mL) was about 2 times higher than that in the model group, with a significant difference (P < 0.05). ROS level decreased significantly by 13% when compared with the model group (677.80). These data suggested a protective effect of AAF on H2O2-indued oxidative damage. Therefore, it can be further developed into a potent natural antioxidant product.

Key words: Actinidia arguta, flavonoids, H2O2-induced oxidative injury, HaCaT cell injury, protective effect

中图分类号:

TS255.1

石浩，王仁才，吴小燕，刘琼. 软枣猕猴桃黄酮对过氧化氢诱导HaCaT细胞损伤的保护作用[J]. 食品科学, 2018, 39(13): 229-234.

SHI Hao, WANG Rencai*, WU Xiaoyan, LIU Qiong. Protective Effect of Actinidia arguta Flavonoid on Hydrogen Peroxide-Induced Injury in HaCaT Cells[J]. FOOD SCIENCE, 2018, 39(13): 229-234.

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软枣猕猴桃黄酮对过氧化氢诱导HaCaT细胞损伤的保护作用

Protective Effect of Actinidia arguta Flavonoid on Hydrogen Peroxide-Induced Injury in HaCaT Cells

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