食品科学 ›› 2018, Vol. 39 ›› Issue (14): 29-35.doi: 10.7506/spkx1002-6630-201814005

• 食品化学 • 上一篇    下一篇

麦芽糖浆糖基化改性玉米醇溶蛋白及在胶囊壳中的应用

张慧君,陈又铭,辛德慧,沙迪昕,王文霞,姜宁宁,郭浩,张慧敏   

  1. (齐齐哈尔大学食品与生物工程学院,黑龙江?齐齐哈尔 161006)
  • 出版日期:2018-07-25 发布日期:2018-07-16
  • 基金资助:
    黑龙江省自然科学基金面上项目(C201455);齐齐哈尔市科技局社会发展攻关项目(SFGG-201605)

Wet-Heating for Glycosylation of Zein with Maltose Syrup and Application in Capsule Shells

ZHANG Huijun, CHEN Youming, XIN Dehui, SHA Dixin, WANG Wenxia, JIANG Ningning, GUO Hao, ZHANG Huimin   

  1. (College of Food and Biological Engineering, Qiqihar University, Qiqihar 161006, China)
  • Online:2018-07-25 Published:2018-07-16

摘要: 利用氨基酸分析仪和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(sodium dodecyl sulfate-polyacrylamide gel electrophoresis,SDS-PAGE)确定提取玉米醇溶蛋白试剂,采用麦芽糖浆通过湿法糖基化方法改性玉米醇溶蛋白制备胶囊壳,对改性产物进行机械性能分析。结果表明:以70%乙醇溶液提取的玉米醇溶蛋白做糖基化反应的氨基供体,当玉米醇溶蛋白-麦芽糖浆质量比为11∶1、pH?9、超声功率400?W、加热时间15?min条件下,接枝度达34.96%,抗拉强度为9.22MPa,约为改性前(4.2MPa)的2?倍。SDS-PAGE糖蛋白染色胶片证明了糖分子和蛋白质分子进行有效的接合。将玉米醇溶蛋白改性产物制成胶囊壳,按照《中华人民共和国药典》(2015版)检测胶囊壳规格外观形态、颜色、尺寸、松紧度、脆碎度、干燥质量损失、灼烧残渣和透明度等性能指标,全部合格。

关键词: 玉米醇溶蛋白, 糖基化, 机械性能, 胶囊壳

Abstract: The main objective of this study was to improve the mechanical properties of zein films by glycosylation for producing plant-derived capsule shells. The results showed that zein, which was extracted with 70% ethanol, was the amino donor of glycosylation by amino acid analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The optimal conditions were determined as follows: ratio between zein and maltose syrup (MS), 11:1; pH, 9; ultrasonic power, 400 W; and time, 15 min. The tensile strength (TS) of the films produced under these conditions was 9.22 MPa, which was increased by about 2-fold compared with that (4.2 MPa) of unmodified zein films, and the degree of glycosylation (DG) was 34.96%. The formation of conjugates was verified by SDS-PAGE with both protein staining and carbohydrate staining. The appearance, shape, color, size, tightness, friability, mass loss during drying and ash content of zein-MS capsule shells met the requirements of the Chinese pharmacopoeia (2015 Edition).

Key words: zein, glycosylation, structural characterization, capsule shells

中图分类号: