1 株携带mcr-1的食品动物源多药耐药奇异变形杆菌耐药性状分析

doi:10.7506/spkx1002-6630-201814022

食品科学 ›› 2018, Vol. 39 ›› Issue (14): 145-150.doi: 10.7506/spkx1002-6630-201814022

1 株携带mcr-1的食品动物源多药耐药奇异变形杆菌耐药性状分析

张德福1,2，安慧1，张健1，赵禹宗1，张蕊1，刘雪飞1，杨立娜1，白凤翎1，李春3，周冬生2，李钰金4，殷喆2，励建荣1,*

（1.渤海大学食品科学与工程学院，生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心，辽宁省食品安全重点实验室，辽宁省高等学校生鲜食品产业技术研究院，辽宁?锦州 121013；2.军事科学院军事医学研究院微生物流行病研究所，病原微生物生物安全国家重点实验室，北京 100071；3.渤海大学数理学院，辽宁?锦州 121013；4.荣成泰祥食品股份有限公司，山东?荣成 264309）

出版日期:2018-07-25 发布日期:2018-07-16
基金资助:
辽宁省自然科学基金项目（20170540022）；辽宁省教育厅项目（LY2016001）；泰山学者蓝色产业领军人才团队支撑计划项目（鲁政办字【2015】19号）

Drug Resistance Analysis of a Multidrug-Resistant Proteus mirabilis Carrying the mcr-1 Gene Isolated from Food-Producing Animals

ZHANG Defu1,2, AN Hui1, ZHANG Jian1, ZHAO Yuzong1, ZHANG Rui1, LIU Xuefei1, YANG Lina1, BAI Fengling1, LI Chun3, ZHOU Dongsheng2, LI Yujin4, YIN Zhe2, LI Jianrong1,*

(1. National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Food Safety Key Lab of Liaoning Province, Fresh Food Storage and Processing Technology Research Institute ofLiaoning Provincial Universities, College of Food Science and Technology, Bohai University, Jinzhou 121013, China;2. State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing100071, China; 3. College of Mathematics and Physics, Bohai University, Jinzhou 121013, China;4. Rongcheng Taixiang Food Co. Ltd., Rongcheng 264309, China)

Online:2018-07-25 Published:2018-07-16

摘要/Abstract

摘要： 在对食品动物源性多药耐药菌的调查过程中，发现一株携带mcr-1的多药耐药奇异变形杆菌F160211，对其耐药表型及基因型进行进一步研究。通过扩增16S rRNA基因对该菌进行鉴定，用纸片扩散法初步鉴定了该菌的耐药谱，在此基础上测定多种药物对其最小抑菌浓度（minimum inhibitory concentration，MIC），用聚合酶链反应法扩增常见的耐药基因，最后分析携带mcr-1的质粒的复制类型。结果表明，该菌为奇异变形杆菌；纸片扩散法和肉汤稀释法测定耐药谱及MIC表明，该菌对青霉素类、第1代和第2代头孢菌素类、大环内酯类、氨基糖苷类、酰胺醇类、四环素类、磺胺类、利福霉素类和黏菌素类等耐药，对呋喃类、多肽类、喹诺酮类中介耐药，对第3代和第4代头孢菌素类、单环β-内酰胺类、碳青霉烯类、甘氨酰环素类敏感；常见耐药基因扩增表明，该菌携带blaCTX-M-1、blaCTX-M-9、blaTEM、blaOXA-1、mph(A)、qnrS和mcr-1等多种耐药基因；mcr-1基因位于与文献报道的pHNSHP45类似的IncI2型不相容性质粒上。本研究表明，mcr-1基因的宿主范围已由报道的大肠杆菌、沙门菌等进一步扩大至奇异变形杆菌，食品动物源性细菌耐药的形势非常严峻，对食品安全和公众健康都是严重的威胁。

关键词: mcr-1, 食品动物, 多药耐药, 最小抑菌浓度, 奇异变形杆菌

Abstract: During the investigation of the prevalence of multidrug-resistant bacteria from food-producing animals, an mcr-1-carring multidrug-resistant strain of Proteus mirabilis was isolated and designated as F160211. Its drug-resistant profile and genotype were further investigated. The isolate was identified by 16S rRNA gene amplification and its drug-resistant profile was defined using a disk-based test and minimum inhibitory concentration (MIC) test. The drug-resistance genes were amplified by polymerase chain reaction (PCR). At last, the replication type of mcr-1-carring plasmid was analyzed. The results showed that the isolate F160211 was identified as P. mirabilis, and it was resistant to penicillin, first- and second-generation cephalosporins, macrolides, aminoglycosides, amphenicols, tetracyclines, sulfanilamides and rifamycins, and was intermediate to furans, polypeptide and quinolones, and was susceptible to third- and fourth-generation cephalosporins, monobactams, carbapenems, glycylcyclines and polymyxins. The drug-resistance genes that F160211 carried included blaCTX-M-1, blaCTX-M-9, blaTEM, blaOXA-1, mph(A), qnrS and mcr-1. The replicon PCR showed that mcr-1 was located in a IncI2 type plasmid, which was similar to pHNSHP45. These results showed that the mcr-1-harboring host bacteria have been extended from Escherichia coli and Salmonella to P. mirabilis and that the grim problem of multidrug-resistant bacteria from food-producing animals will pose a great threat to food safety and public health.

Key words: mcr-1, food-producing animals, multidrug resistance, minimum inhibitory concentration (MIC), Proteus mirabilis

中图分类号:

TS201.3

张德福，安慧，张健，赵禹宗，张蕊，刘雪飞，杨立娜，白凤翎，李春，周冬生，李钰金，殷喆，励建荣. 1 株携带mcr-1的食品动物源多药耐药奇异变形杆菌耐药性状分析[J]. 食品科学, 2018, 39(14): 145-150.

ZHANG Defu, AN Hui ZHANG Jian ZHAO Yuzong ZHANG Rui LIU Xuefei YANG Lina BAI Fengling LI Chun, ZHOU Dongsheng, LI Yujin, YIN Zhe, LI Jianrong. Drug Resistance Analysis of a Multidrug-Resistant Proteus mirabilis Carrying the mcr-1 Gene Isolated from Food-Producing Animals[J]. FOOD SCIENCE, 2018, 39(14): 145-150.

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1 株携带mcr-1的食品动物源多药耐药奇异变形杆菌耐药性状分析

Drug Resistance Analysis of a Multidrug-Resistant Proteus mirabilis Carrying the mcr-1 Gene Isolated from Food-Producing Animals

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