食品科学 ›› 2018, Vol. 39 ›› Issue (15): 190-196.doi: 10.7506/spkx1002-6630-201815028

• 包装贮运 • 上一篇    下一篇

根皮苷对苹果采后灰霉病的影响

贺军花,马利菁,周会玲*   

  1. 西北农林科技大学园艺学院,陕西 杨凌 712100
  • 出版日期:2018-08-15 发布日期:2018-08-15
  • 基金资助:
    现代农业产业技术体系建设专项(nycylx-08-05-02)

Effect of Phlorizin Treatment on Postharvest Gray Mold of Apples

HE Junhua, MA Lijing, ZHOU Huiling*   

  1. College of Horticulture, Northwest A & F University, Yangling 712100, China
  • Online:2018-08-15 Published:2018-08-15

摘要: 为探究苹果特有酚类物质根皮苷对灰霉病的影响,以培养基体外接种灰霉菌确定抑菌效果,预实验筛选得 到活体实验的最佳质量浓度。以此质量浓度根皮苷溶液浸泡处理‘粉红女士’苹果20 min,诱导12 h,损伤接种灰 霉孢,定期统计发病情况并取样测定抗性相关指标,以清水浸泡为对照。结果表明:根皮苷可直接抑制灰霉菌的体 外生长,1.0 g/L的根皮苷处理可显著降低采后果实的发病率和抑制病斑直径的扩展(P<0.05),并诱导果肉苯丙 氨酸解氨酶、过氧化物酶和多酚氧化酶活力的升高,促进总酚、类黄酮和木质素的积累,同时降低了H2O2的含量, 果实抗性提高。结果可为根皮苷对采后病害抗性的机理研究提供理论依据。

关键词: 根皮苷, 苹果, 灰霉病, 诱导抗性

Abstract: In order to explore the effect of phlorizin, a phenolic compound uniquely found in apples, on gray mold, the antifungal effect was determined by inoculation of Botrytis cinerea in vitro. The best concentration of phlorizin for in vivo test was screened and used to soak ‘Pink Lady’ apples for 20 min, followed by induction for 12 h, and then B. cinerea was inoculated. The incidence of resistance was measured and resistance-related indexes were determined regularly. Distilled water treatment was used as the control group. The results showed that phloridzin could directly inhibit the growth of B. cinerea in vitro. Phlorizin at 1.0 g/L could significantly reduce the incidence of postharvest gray mold and inhibit the expansion of lesion diameter (P < 0.05). It could also increase the activity of phenylalanine ammonialyase, peroxidase and polyphenol oxidase, and promote the accumulation of total phenols, total flavonoids and lignin. At the same time, it could reduce the content of hydrogen peroxide and increase fruit resistance. These results can provide the theoretical basis for the study on the mechanisms of postharvest diseases in apples.

Key words: phlorizin, apple, gray mold, induced resistance

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