食品科学 ›› 2018, Vol. 39 ›› Issue (17): 127-133.doi: 10.7506/spkx1002-6630-201817021

• 营养卫生 • 上一篇    下一篇

茶花粉的抗氧化活性及其对急性酒精肝损伤的保护作用

彭国霞1,赵浩安1,刘清清1,张 颖1,程 妮1,2,曹 炜1,2,*   

  1. 1.西北大学食品科学与工程学院,陕西 西安 710069;2.陕西省蜂产品工程技术研究中心,陕西 西安 710065
  • 出版日期:2018-09-15 发布日期:2018-09-18
  • 基金资助:
    国家自然科学基金面上项目(31272510);陕西省社会发展攻关项目(2016SF-425);西安市农业技术研发项目(NC1405(1));陕西省教育厅产业化培育项目(16JF025)

Antioxidant and Hepatoprotective Effects of Camellia japonica Bee Pollen on Acute Alcohol-Induced Liver Damage in Mice

PENG Guoxia1, ZHAO Haoan1, LIU Qingqing1, ZHANG Ying1, CHENG Ni1,2, CAO Wei1,2,*   

  1. 1. College of Food Science and Engineering, Northwest University, Xi’an 710069, China; 2. Bee Product Research Center of Shaanxi Province, Xi’an 710065, China
  • Online:2018-09-15 Published:2018-09-18

摘要: 本实验测定了5 个产地茶花粉的总酚和总黄酮含量,在此基础上,研究了茶花粉的体外抗氧化活性及 其对酒精诱导的急性肝损伤的保护作用。结果表明,浙江茶花粉的总酚和总黄酮含量最高,分别为8.97 mg/g 和3.01 mg/g,并且具有较高的抗氧化活性。给小鼠连续灌胃茶花粉7 周,小鼠血清氧自由基吸收能力显著增 加(P<0.05),Cu2+诱导的脂质过氧化损伤显著改善(P<0.05)。与模型组相比,茶花粉组小鼠血清中谷草 转氨酶和谷丙转氨酶活力显著下降(P<0.05),肝脏组织中超氧化物歧化酶和谷胱甘肽过氧化物酶活力显著 上升(P<0.05),丙二醛含量显著降低(P<0.05)。组织病理学观察显示,茶花粉能够明显改善酒精引起的肝细 胞损伤。本研究结果表明茶花粉不仅具有较强的抗氧化活性,还对酒精造成的急性肝损伤有明显的保护作用,可以 作为一种保护肝脏损伤的功能性食品原料。

关键词: 茶花粉, 总酚, 总黄酮, 抗氧化, 保护肝脏

Abstract: In the present study, Camellia japonica bee pollen from five geographical origins in China were analyzed for total phenolic and total flavonoid contents. We also evaluated the antioxidant capacity and hepatoprotective effects of C. japonica bee pollen on acute alcohol-induced liver damage in mice. The results showed that C. japonica bee pollen from Zhejiang province exhibited the highest total phenolic (8.97 mg/g calculated as gallic acid equivalent) and total flavonoid content (3.01 mg/g calculated as rutin equivalent) and strong antioxidant capacity. Oral administration of mice with crude C. japonica bee pollen for 7 consecutive weeks significantly increased serum oxygen radical absorbance capacity (P < 0.05), lowered the levels of aspartate aminotransferase and alanine aminotransferase as well as lipoprotein oxidation in serum (P < 0.05), enhanced the activities of glutathione peroxidase and superoxide dismutase in liver (P < 0.05), and decreased the formation of malondialdehyde in liver (P < 0.05). Histopathological examinations showed that C. japonica bee pollen is efficacious for the prevention and treatment of alcohol-induced hepatic injury in mice. This study concluded that C. japonica bee pollen exhibits hepatoprotective properties and may be used as an ingredient of functional foods.

Key words: Camellia japonica bee pollen, total polyphenols, total flavonoids, antioxidant, hepatoprotective

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