食品科学 ›› 2018, Vol. 39 ›› Issue (20): 119-125.doi: 10.7506/spkx1002-6630-201820018

• 生物工程 • 上一篇    下一篇

不同培养温度下腐败希瓦氏菌模式菌株DSM6067差异蛋白质组分析

杨胜平,钱韻芳*,章缜,程颖,谢晶*   

  1. (上海海洋大学食品学院,上海水产品加工及贮藏工程技术研究中心,上海 201306)
  • 出版日期:2018-10-25 发布日期:2018-10-24
  • 基金资助:
    国家自然科学基金面上项目(31571914);上海市科技兴农重点攻关项目(沪农科攻字(2015)第4-12号); 上海市科委工程中心能力提升项目(16DZ2280300)

Comparative Proteome Analysis of Shewanella putrefaciens DSM6067 Cultured at Different Temperatures

YANG Shengping, QIAN Yunfang*, ZHANG Zhen, CHENG Ying, XIE Jing*   

  1. (Shanghai Engineering Research Center of Aquatic Product Processing & Preservation, College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306, China)
  • Online:2018-10-25 Published:2018-10-24

摘要: 腐败希瓦氏菌能够适应低温环境继续生长繁殖,是导致冷链流通过程中水产品腐败变质的优势腐败菌之一。运用Label-free非标记蛋白质组学技术对30、10?℃和4?℃培养温度下腐败希瓦氏菌模式菌株DSM6067所表达的蛋白质组差异进行分析,以期为探明腐败希瓦氏菌在低温胁迫下的分子调控机制提供理论依据。结果表明,腐败希瓦氏菌生长的环境温度越低,其所表达的蛋白质种类数越多。检测分析共得到2?165?条腐败希瓦氏菌蛋白质,其中30、10?℃和4?℃鉴定到的蛋白质条数分别为1?739、1?761和1?832。功能分析表明,这些差异蛋白涉及多种GO分类及KEGG通路,其中与能量代谢、催化活性、分子绑定功能相关的GO分类占较大比例。在这些差异蛋白中,与腐败希瓦氏菌模式菌株细胞膜脂肪酸合成相关的蛋白表达量相对较低,但4、10?℃与30?℃培养温度下相比差异较显著,且温度越低与细胞膜脂肪酸代谢相关的蛋白酶表达量越高。本研究为进一步探析腐败希瓦氏菌野生菌株适冷机制提供理论参考依据。

关键词: 温度, 腐败希瓦氏菌, 蛋白质组学, GO分类, KEGG通路

Abstract: Shewanella putrefaciens is a cold-adapted microorganism, which is considered as one of the most important species causing spoilage of sea foods. This study aimed to elucidate the molecular mechanism of the response to cold stress in S. putrefaciens. The differential expression of the proteome in S. putrefaciens DSM6067 at different temperatures (30, 10 and 4 ℃) was investigated using a label-free quantitative proteomic approach and two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS). The results showed that more proteins were expressed when the strain was cultured at lower temperatures. A total of 2 165 protein bands were obtained, 1 739, 1 761 and 1 832 of them being identified at 30,10 and 4 ℃, respectively. Bioinformatics analysis revealed that these proteins participated in several gene ontology (GO) categories and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. According to GO analysis, the functional categories were dominated by metabolic activity, catalytic activity, and molecular binding. Although the expression levels of proteins participated in fatty acid metabolism pathway were relatively lower in all samples among the whole metabolic pathways, there were significant differences in protein expression at different culture temperatures and the lower the temperature, the higher the expression level.

Key words: temperature, Shewanella putrefaciens, proteomics, GO categories, KEGG pathways

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