基于稳定同位素和矿质元素的中华绒螯蟹产地鉴别潜力评价

doi:10.7506/spkx1002-6630-20190109-108

食品科学 ›› 2020, Vol. 41 ›› Issue (2): 298-305.doi: 10.7506/spkx1002-6630-20190109-108

基于稳定同位素和矿质元素的中华绒螯蟹产地鉴别潜力评价

骆仁军，姜涛，陈修报，薛竣仁，刘洪波，杨健

（1.南京农业大学无锡渔业学院，江苏无锡 214081；2.中国水产科学研究院长江中下游渔业生态环境评价与资源养护重点实验室，淡水渔业研究中心，江苏无锡 214081）

出版日期:2020-01-25 发布日期:2020-01-19
基金资助:
国家自然科学基金面上项目（31772850）；“十二五”国家科技支撑计划项目（2015BAD17B05）

Potential Assessment of Stable Isotopes and Mineral Elements for Geographic Origin Identification of Eriocheir sinensis

LUO Renjun, JIANG Tao, CHEN Xiubao, XUE Junren, LIU Hongbo, YANG Jian

(1. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; 2. Key Laboratory of Fishery Ecological Environment Assessment and Resource Conservation in Middle and Lower Reaches of the Yangtze River, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China)

Online:2020-01-25 Published:2020-01-19

摘要/Abstract

摘要： 为研究中华绒螯蟹的产地溯源，对黄河和辽河3 个产地的中华绒螯蟹进行采样，结合稳定同位素比、矿质元素分析以及化学计量学方法分析和探讨不同产地之间差异因子和分类模型。单因素方差分析及Duncan多重比较检验结果显示，在这3 个产地间，元素Na、Mg、Al、K、Zn、Sr、Ba以及同位素δ13C和δ15N含量在产地间有显著差异（P＜0.05）或极显著差异（P＜0.01），而元素Ca、Mn、Cu在不同产地螯蟹中的含量差异不明显（P＞0.05）。自组织映射神经网络聚类分析可视化显示，单独利用矿质元素或结合利用稳定同位素和矿质元素的最佳聚类效果均将位于黄河口的山东东营中华绒螯蟹样本聚为一类，辽河的盘锦和营口的样本聚为另一类。线性判别分析的结果显示，结合利用稳定同位素和矿质元素或单独利用矿质元素的初始验证的正确率均高于95%，且稳定同位素和矿质元素的结合具有更高的产地区分潜力。

关键词: 中华绒螯蟹, 稳定同位素, 矿质元素, 自组织映射, 线性判别分析, 产地溯源

Abstract: In the present study, chemometrics profiles of mineral elements and stable isotopes for Chinese mitten crabs (Eriocheir sinensis) from three geographic origins in China: the Yellow River (one location) and Liaohe River (two locations) were analyzed and compared with each other to assess the potential of the mineral elements and stable isotopes for geographic origin identification. The results showed that significant (P < 0.05) or highly significant (P < 0.01) differences in the contents of 7 elements (Na, Mg, Al, K, Zn, Sr, and Ba) and two stable isotopes (δ13C and δ15N) in crabs were found among locations, despite no significant differences (P > 0.05) in the contents of Ca, Mn and Cu. Nevertheless, the self-organizing map analysis of the mineral elements and stable isotope profiles visually clustered the two locations of Liaohe River into one group, which was distinguished from the remaining group of crabs from Dongying, located on the estuary of the Yellow River. Linear discriminant analysis showed that the accuracies of initial verification with the mineral elements alone and in combination with the stable isotopes were both higher than 95%, and, moreover, the combination of mineral elements and stable isotopes had an even higher potential for geographic origin identification.

Key words: Eriocheir sinensis, stable isotopes, mineral elements, self-organizing map, linear discriminant analysis, geographic origin discrimination

中图分类号:

S912

骆仁军，姜涛，陈修报，薛竣仁，刘洪波，杨健. 基于稳定同位素和矿质元素的中华绒螯蟹产地鉴别潜力评价[J]. 食品科学, 2020, 41(2): 298-305.

LUO Renjun, JIANG Tao, CHEN Xiubao, XUE Junren, LIU Hongbo, YANG Jian. Potential Assessment of Stable Isotopes and Mineral Elements for Geographic Origin Identification of Eriocheir sinensis[J]. FOOD SCIENCE, 2020, 41(2): 298-305.

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基于稳定同位素和矿质元素的中华绒螯蟹产地鉴别潜力评价

Potential Assessment of Stable Isotopes and Mineral Elements for Geographic Origin Identification of Eriocheir sinensis

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