高效液相色谱-串联质谱法检测动物源性食品中异丙嗪

doi:10.7506/spkx1002-6630-20190115-166

食品科学 ›› 2019, Vol. 40 ›› Issue (24): 320-324.doi: 10.7506/spkx1002-6630-20190115-166

高效液相色谱-串联质谱法检测动物源性食品中异丙嗪

李志刚，李慧晨，马燕红，王守伟，任南，郭文萍，郭超，李莹莹

（中国肉类食品综合研究中心，北京 100068）

出版日期:2019-12-25 发布日期:2019-12-24
基金资助:
“十三五”国家重点研发计划重点专项（2017YFE0110800）

High Performance Liquid Chromatography-Tandem Mass Spectrometry Detection of Promethazine in Animal-Derived Foods

LI Zhigang, LI Huichen, MA Yanhong, WANG Shouwei, REN Nan, GUO Wenping, GUO Chao, LI Yingying

(China Meat Research Center, Beijing 100068, China)

Online:2019-12-25 Published:2019-12-24

摘要/Abstract

摘要： 建立高效液相色谱-串联质谱法测定畜肉及内脏中异丙嗪残留量的定量检测方法。首先通过甲酸-乙腈进行试样提取，然后经HLB离子交换萃取小柱净化，最后采用多反应监测模式进行定量分析。结果表明：本方法在0.5～50 ng/mL范围内线性关系良好，线性相关系数大于0.999，检出限和定量限分别为0.1 μg/kg和0.3 μg/kg；基质添加回收范围在85.7%～102.0%之间，相对标准偏差在0.9%～7.4%之间（n=6）。该方法前处理简单、快速，可用于畜肉和内脏中异丙嗪的定量测定。

关键词: 异丙嗪, 镇静剂, 高效液相色谱-串联质谱法

Abstract: In this research, an analytical method for the determination of promethazine residues in livestock meat and viscera by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was established. Samples were extracted with formic acid-acetonitrile, and then the extract was purified on an HLB ion exchange extraction column before being quantitatively analyzed by using multi-reaction monitoring (MRM) mode. The results showed that the method had a good linear relationship in the concentration range of 0.5–50 ng/mL with a correlation coefficient of greater than 0.999. The limit of detection (LOD) and limit of quantitation (LOQ) were 0.1 and 0.3 μg/kg, respectively. The recoveries from spiked samples were 85.7%–102.0%, and the relative standard deviations for precision were 0.9%–7.4% (n = 6). This method is simple and rapid, and can be used for the determination of promethazine in animal-derived foods.

Key words: promethazine, sedative, high performance liquid chromatography-tandem mass spectrometry

中图分类号:

TS201.6

李志刚，李慧晨，马燕红，王守伟，任南，郭文萍，郭超，李莹莹. 高效液相色谱-串联质谱法检测动物源性食品中异丙嗪[J]. 食品科学, 2019, 40(24): 320-324.

LI Zhigang, LI Huichen, MA Yanhong, WANG Shouwei, REN Nan, GUO Wenping, GUO Chao, LI Yingying. High Performance Liquid Chromatography-Tandem Mass Spectrometry Detection of Promethazine in Animal-Derived Foods[J]. FOOD SCIENCE, 2019, 40(24): 320-324.

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高效液相色谱-串联质谱法检测动物源性食品中异丙嗪

High Performance Liquid Chromatography-Tandem Mass Spectrometry Detection of Promethazine in Animal-Derived Foods

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