食品科学 ›› 2020, Vol. 41 ›› Issue (6): 93-100.doi: 10.7506/spkx1002-6630-20190217-083

• 生物工程 • 上一篇    下一篇

新疆喀什地区维吾尔族婴幼儿肠道双歧杆菌遗传差异及益生特性分析

魏小晶,周桓丞,靳亚梅,蔡静静,倪永清,张艳   

  1. (石河子大学食品学院,新疆 石河子 832003)
  • 出版日期:2020-03-25 发布日期:2020-03-23
  • 基金资助:
    国家自然科学基金地区科学基金项目(31760446);石河子大学高层次人才启动项目(CZX201432R)

Genetic Diversity and Probiotic Characteristics of Intestinal Bifidobacterium Strains Isolated from Uygur Infants in Kashi, Xinjiang

WEI Xiaojing, ZHOU Huancheng, JIN Yamei, CAI Jingjing, NI Yongqing, ZHANG Yan   

  1. (Food College, Shihezi University, Shihezi 832003, China)
  • Online:2020-03-25 Published:2020-03-23

摘要: 分析新疆喀什地区维吾尔族婴幼儿肠道双歧杆菌的遗传差异,以期为少数民族婴幼儿肠道益生双歧杆菌的开发奠定一定基础。本实验采用16S rRNA基因序列结合基因外重复回文序列聚合酶链式反应指纹分型技术,对菌株遗传结构差异进行分析,根据遗传指纹挑选部分代表性双歧杆菌,检测它们对常见6 种致病菌的抑菌活性并对抑菌性能较好的菌株进行耐酸耐胆盐、碳源代谢及抗生素药敏实验。研究发现有75 株为双歧杆菌,隶属于4 个种及2 个亚种,分别为Bifidobacterium bifidum、B. pseudocatenulatum、B. adolescentis、B. longum subsp. infantis和B. longum subsp. suis。抑菌实验结果表明,有16 株双歧杆菌具有广谱抑菌性能;药敏实验显示这些菌株除对阿米卡星及万古霉素表现耐药外,对其他抗生素均表现敏感或中度敏感;耐酸耐胆盐实验表明抑菌菌株中B. longum subsp. infantis f65-26和B. pseudocatenulatum f115-8为耐受性最优菌株,具有潜在开发利用价值。

关键词: 婴幼儿肠道, 双歧杆菌, 16S rRNA, 指纹图谱, 益生特性, 抗生素敏感性

Abstract: The purpose of this study was to analyze the genetic and species heterogeneity of bifidobacterial strains isolated from the intestinal tract of Uygur infants in Kashi, Xinjiang, and, more broadly, to lay the foundation for exploiting intestinal probiotic bifidobacteria resources from ethnic minority infants. The inter-strain difference in genetic structure was analyzed according to the 16S rRNA gene sequence using rep-PCR fingerprint typing technology. According to the genetic fingerprints, some representative bifidobacteria were selected and tested for their antibacterial activities against 6 common pathogenic bacteria, and the strains with good antibacterial activity were further tested for acid and bile resistance, carbon source metabolism and antibiotic susceptibility. This study found that the 75 strains of Bifidobacterium were assigned to four species and two subspecies, B. bifidum, B. pseudocatenulatum, B. adolescentis, B. longum subsp. infantis and B. longum subsp. suis, respectively. The bacteriostatic tests showed that 16 of the 75 strains had broad-spectrum antibacterial properties. Drug sensitivity test showed that these strains were sensitive or moderately sensitive to all antibiotics except amikacin and vancomycin. The acid and bile salt tolerance tests showed that B. longum subsp. infantis f65-26 and B. pseudocatenulatum f115-8 were the most tolerant strains, which could have great potential for development and utilization.

Key words: infant intestine, Bifidobacteria, 16S rRNA gene sequence, fingerprints, probiotic characteristics, antibiotics resistance

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