食品科学 ›› 2019, Vol. 40 ›› Issue (18): 323-328.doi: 10.7506/spkx1002-6630-20190220-120

• 安全检测 • 上一篇    下一篇

基于钴金属有机骨架材料(ZIF-67)催化性能的牛奶中大肠杆菌O157:H7快速定量检测

王淑娟,刘程,方水琴,田亚晨,董庆利,王翔,许东坡,刘箐   

  1. (1.上海理工大学医疗器械与食品学院,上海 200093;2.青岛海洋科学与技术试点国家实验室,海洋渔业科学与食物产出过程功能实验室,山东?青岛 266071)
  • 出版日期:2019-09-25 发布日期:2019-09-23
  • 基金资助:
    上海市科技创新行动计划项目(18495800400);上海市农委科技兴农项目(沪农科推字(2017)第4-4号)

Rapid Quantitative Detection of Escherichia coli O157:H7 in Milk Based on Catalytic Activity of Cobalt-Based Metal Organic Framework (ZIF-67)

WANG Shujuan, LIU Cheng, FANG Shuiqin, TIAN Yachen, DONG Qingli, WANG Xiang, XU Dongpo, LIU Qing,   

  1. (1. School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China; 2. Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266071, China)
  • Online:2019-09-25 Published:2019-09-23

摘要: 建立一种基于金属有机骨架催化性能的快速定量检测牛奶中大肠杆菌O157:H7的方法。以2-甲基咪唑和CoCl2?6H2O为原料,Bola型两亲性表面活性剂为溶剂合成一种钴金属有机骨架材料(ZIF-67)。对ZIF-67的催化性能进行研究,发现ZIF-67具有和辣根过氧化物酶类似的催化活性,并能在无过氧化氢存在的情况下催化3,3’,5,5’-四甲基联苯胺显色。基于此建立一种通过磁性微球进行特异性富集,通过检测与靶标结合的ZIF-67的量间接检测牛奶中大肠杆菌O157:H7的方法,该方法的检测范围为17~1.7×108?CFU/mL,检测限为17?CFU/mL,检测过程在1?h以内。该方法能快速、简单、高灵敏度、高选择性定量地检测出牛奶中的大肠杆菌O157:H7,在食源性致病菌的快速筛查中具有重要的应用价值。

关键词: 钴金属有机骨架(ZIF-67)材料, 过氧化物酶样活性, 显色反应, 双抗体夹心法, 大肠杆菌O157:H7

Abstract: The aim of this study was to establish a new method for the rapid quantitative detection of Escherichia coli O157:H7 (E. coli O157:H7) in milk samples based on the catalytic activity of metal-organic framework. A cobalt-based metal organic framework (ZIF-67) was synthesized by using 2-methylimidazole and cobalt chloride hexahydrate as starting materials with Bola amphiphilic surfactant as solvent. The catalytic performance of ZIF-67 was studied. It was found that ZIF-67 had similar catalytic activity to horseradish peroxidase (HRP) and could catalyze the chromogenic reaction of 3,3’,5,5’-tetramethylbenzidine (TMB) in the absence of hydrogen peroxide (H2O2). Based on this, we established a method for indirect detection of E. coli O157:H7 in milk by specific enrichment with magnetic microspheres and subsequent detection of ZIF-67 combined with the target. The detection range of this method was 17–1.7 × 108 CFU/mL, and the detection limit (LOD) was 17 CFU/mL. The detection took 1 h. This method could detect E. coli O157:H7 in milk quickly and simply with high sensitivity and selectivity. It has great potential for application in rapid screening of foodborne pathogens.

Key words: cobalt-based metal organic framework (ZIF-67), peroxidase-like activity, chromogenic reaction, double antibody sandwich method, Escherichia coli O157:H7

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