食品科学 ›› 2019, Vol. 40 ›› Issue (21): 136-142.doi: 10.7506/spkx1002-6630-20190318-217

• 营养卫生 • 上一篇    下一篇

硫酸化羊肚菌多糖调控胆固醇代谢作用

唐瑜婉,张月巧,李瑶,雷琳,李富华,赵吉春,吴素蕊,明建   

  1. (1.西南大学食品科学学院,重庆 400715;2.西南大学食品贮藏与物流研究中心,重庆 400715;3.中华全国供销合作总社昆明食用菌研究所,云南 昆明 650223)
  • 出版日期:2019-11-15 发布日期:2019-12-02
  • 基金资助:
    国家自然科学基金面上项目(31271825;31471576)

Regulation of Cholesterol Metabolism by Sulfated Polysaccharides from Morchella angusticeps Peck

TANG Yuwan, ZHANG Yueqiao, LI Yao, LEI Lin, LI Fuhua, ZHAO Jichun, WU Surui, MING Jian   

  1. (1. College of Food Science, Southwest University, Chongqing 400715, China; 2. Research Center of Food Storage & Logistics, Southwest University, Chongqing 400715, China; 3. Kunming Edible Fungi Institute, All China Federation of Supply and Marketing Cooperatives, Kunming 650223, China)
  • Online:2019-11-15 Published:2019-12-02

摘要: 黑脉羊肚菌多糖(polysaccharides from Morchella angusticeps Peck,PMEP)已被证实可以降低胆固醇。本研究旨在比较PMEP及其硫酸化多糖(sulfated PMEP,SPMEP)对高胆固醇饮食大鼠的降胆固醇活性。结果表明:高剂量的PMEP和SPMEP可使大鼠的血清总胆固醇质量浓度分别降低15%和23%,表明SPMEP比PMEP具有更强的降胆固醇活性。PMEP和SPMEP降低血清胆固醇质量浓度伴随着肝脏3-羟基-3-甲基戊二酰CoA还原酶表达显著下调及7α-羟化酶表达显著上调(P<0.05)。此外,PMEP和SPMEP都可以显著降低肝脏胆固醇质量浓度(P<0.05),同时显著提高粪便和肠道胆汁酸的总量(P<0.05)。结论:PMEP通过硫酸化修饰可以增强对大鼠的降胆固醇活性。

关键词: 黑脉羊肚菌, 多糖, 硫酸化修饰, 胆固醇代谢

Abstract: Polysaccharide extracted from Morchella angusticeps Peck (PMEP) has been proved to have a hypocholesterolemic effect. This study aimed to compare the cholesterol-lowering activity of PMEP and its sulfated derivative (SPMEP) in rats fed on a high-cholesterol diet. Results showed that PMEP at high dose and SPMEP could decrease serum total cholesterol by 15% and 23%, respectively, indicating that SPMEP had a stronger cholesterol-lowering potency than PMEP (P < 0.05). PMEP and SPMEP down-regulated the expression of hepatic 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase and up-regulated the expression of cholesterol-7α-hydroxylase (P < 0.05). Besides, both of them could decrease liver cholesterol, while significantly increasing the excretion of total fecal and intestinal bile acids (P < 0.05). Therefore, the cholesterol-lowering activity of PMEP in rats can be enhanced after being sulfated.

Key words: Morchella angusticepes Peck, polysaccharide, sulfation, cholesterol metabolism

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