食品科学 ›› 2020, Vol. 41 ›› Issue (8): 116-122.doi: 10.7506/spkx1002-6630-20190411-138

• 生物工程 • 上一篇    下一篇

乳酸菌代谢物中二肽基肽酶-4抑制剂的分离纯化及鉴定

董杰,刘鹭,甲承立,朱俊玲,吕加平   

  1. (1.山西农业大学食品科学与工程学院,山西 晋中 030800;2.北京市营养源研究所系统营养工程技术研究中心,北京 100069;3.中国农业科学院农产品加工研究所,北京 100193)
  • 出版日期:2020-04-25 发布日期:2020-04-20
  • 基金资助:
    中央级公益性科研院所基本科研业务费专项 (S2016JC01)

Isolation, Purification and Identification of Dipeptidyl Peptidase-4 Inhibitor from Lactic Acid Bacterial Metabolite

DONG Jie, LIU Lu, JIA Chengli, ZHU Junling, Lü Jiaping   

  1. (1. College of Food Science and Engineering, Shanxi Agriculture University, Jinzhong 030800, China;2. Engineering Research Centre of System-Nutrition, Beijing Institute of Nutrition Resources, Beijing 100069, China;3. Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China)
  • Online:2020-04-25 Published:2020-04-20

摘要: 筛选出具有抑制二肽基肽酶-4(dipeptidyl peptidase-4,DPP-4)活性的植物乳杆菌BLP12,将其代谢物通过有机溶剂萃取、超滤膜、凝胶色谱和高效液相色谱等一系列方法进行分离纯化,利用底物N-甘氨酰脯氨酰-对硝基苯胺盐酸盐与DPP-4在波长405 nm左右强烈的光吸收测定DPP-4活性,选择抑制活性强的部分用液相色谱-质谱联用仪鉴定样品中的物质,质谱共鉴定出61 种物质,其中十二烷基硫酸盐是已有报道的DPP-4抑制剂。

关键词: 乳酸菌, 代谢物, 二肽基肽酶-4, 抑制活性

Abstract: In this study, the metabolites of Lactobacillus plantarum BLP12 with inhibitory activity against dipeptidyl peptidase-4 (DPP-4) were separated and purified by organic solvent extraction, ultrafiltration membrane, gel chromatography and high performance liquid chromatography. The substrate N-glycyl-prolyl-p-nitroanilide hydrochloride (Gly-Pro-pNA) was applied to determine DPP-4 activity based on the strong absorption at around 405 nm of the degradation product of Gly-Pro-pNA. The fraction with strong inhibitory activity was selected for chemical composition analysis by liquid chromatography tandem mass spectrometry. A total of 61 substances were identified including dodecyl sulfate, which has been reported as a DPP-4 inhibitor.

Key words: lactic acid bacteria, metabolite, dipeptidyl peptidase-4, inhibition activity

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