食品科学 ›› 2020, Vol. 41 ›› Issue (9): 66-73.doi: 10.7506/spkx1002-6630-20190412-176

• 营养卫生 • 上一篇    下一篇

白酒中两种萜烯类化合物的细胞内抗氧化活性

张倩,朱婷婷,黄明泉,魏金旺,吴继红,霍嘉颖   

  1. (1.食品质量与安全北京实验室,北京食品营养与人类健康高精尖创新中心,北京市食品风味化学重点实验室,北京工商大学,北京 100048;2.北京顺鑫农业股份有限公司牛栏山酒厂,北京 101301)
  • 出版日期:2020-05-15 发布日期:2020-05-15
  • 基金资助:
    国家自然科学基金面上项目(31871749);2019年研究生科研能力提升计划项目; 酒类风味品质与安全科研创新团队项目(PXM2019-014213-000007)

Intracellular Antioxidant Activity of Two Terpenoids in Baijiu

ZHANG Qian, ZHU Tingting, HUANG Mingquan, WEI Jinwang, WU Jihong, HUO Jiaying   

  1. (1. Beijing Laboratory of Food Quality and Safety, Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Key Laboratory of Flavor Chemistry, Beijing Technology and Business University, Beijing 100048, China; 2. Beijing Shunxin Agriculture Co. Ltd. Niulanshan Distillery, Beijing 101301, China)
  • Online:2020-05-15 Published:2020-05-15

摘要: 利用顶空固相微萃取结合气相色谱质谱联用技术对13 种牛栏山二锅头原酒及3 种商品酒中的萜烯化合物进行定性定量分析,经质谱、外标物比对共定性出5 种萜烯化合物,其中香叶基丙酮(geranylacetone,GAT)在二锅头酒中为首次发现;同时,利用2,2’-偶氮双(2-甲基丙脒)二盐酸盐诱导的HepG2细胞氧化损伤模型探究GAT和β-石竹烯(β-caryophllene,BCP)的抗氧化活性。研究发现,GAT和BCP在牛栏山二锅头白酒中的剂量水平下,对氧化损伤细胞内的活性氧具有清除作用,主要通过增强细胞内抗氧化酶(超氧化物歧化酶、谷胱甘肽过氧化物酶、过氧化氢酶)的活性,提高机体内非酶系统抗氧化剂谷胱甘肽的浓度来实现,同时还可以防止脂质过氧化终产物丙二醛含量的增加,从而提高细胞的抗氧化能力,其抗氧化能力呈浓度依赖性增加。本研究可为白酒的健康功效和白酒行业健康发展提供数据。

关键词: 牛栏山二锅头白酒, 香叶基丙酮, β-石竹烯, 顶空固相微萃取, 抗氧化活性

Abstract: Headspace solid phase microextraction coupled with gas chromatography-mass spectrometry was applied for the qualification and quantification of terpenoids in 13 base Baijius and 3 commercial Baijius of Niulanshan “Erguotou”. Totally 5 terpenoids were identified by the external standard method, including β-caryophllene (BCP) and geranylacetone (GAT), which was first discovered in Erguotou Baijiu. The protective effects of GAT and BCP on oxidative damage induced by 2,2’-azobis(2-methylpropanimidamidine)dihydrochloride (AAPH) in HepG2 cells were investigated. The results showed that at the same concentration as in Erguotou, both GAT and BCP had a potent scavenging effect against intracellular reactive oxygen species by increasing the activity of intracellular antioxidant enzymes including catalase, superoxide dismutase and glutathione peroxidase and the level of non-enzymatic antioxidants such as glutathione (GSH). In addition, they could decrease the level of malondialdehyde (MDA), thereby enhancing cellular antioxidant defense capacity in a dose-dependent manner. This study provides fundamental data supporting the health benefits of Baijiu and the healthy development of the Baijiu industry.

Key words: Niulanshan Erguotou Baijiu, geranylacetone, β-caryophllene, headspace-solid phase microextraction, antioxidant activity

中图分类号: