• 食品工程 •

### 射流空化对大豆11S球蛋白结构和功能特性的影响

1. （东北农业大学食品学院，黑龙江 哈尔滨 150030）
• 出版日期:2020-05-15 发布日期:2020-05-15
• 基金资助:
山东省禹城市第一批“大禹英才”（创新B类）项目；黑龙江省自然科学基金项目（C2018024）； 黑龙江省普通本科高等学校青年创新人才培养计划项目（NYPYSCT-2018163）； 中国博士后科学基金面上资助项目（2018M641798）

### Effect of Jet Cavitation on the Structure and Functional Properties of Soybean 11S Globulin

XIE Changyuan, WANG Zhongjiang, GUO Zengwang, ZHAI Yuyu, TENG Fei, LI Yang

1. (School of Food Science, Northeast Agricultural University, Harbin 150030, China)
• Online:2020-05-15 Published:2020-05-15

Abstract: The effect of different durations of jet cavitation treatment on the structural (fluorescence spectrum, Fourier transform infrared spectrum, surface hydrophobicity, and the contents of carbonyl groups, sulfhydryl groups and disulfide bonds) and functional properties (solubility, emulsifying properties, and foaming properties) of soybean 11S globulin at different concentrations was investigated. The results indicated that with the prolongation of jet cavitation treatment time, the maximum absorption wavelength (λmax) of 11S globulin at concentrations of 2 and 5 g/100 mL showed a red shift first and then a blue shift (the higher the concentration, the higher the λmax), and the fluorescence intensity increased first and then decreased. The α-helix and β-sheet structure of 11S globulin at 2 g/100 mL were converted into β-turn. Toward the end of the treatment, β-turn was converted into α-helix, and the content of random coils did not change significantly during the entire treatment period; at 5 g/100 mL, the α-helix, β-sheet and β-turn were transformed into a random coil structure, and toward the end, the secondary structure was transformed from random coil and β-turn to α-helix and β-sheet. At both concentrations, the surface hydrophobicity and the contents of carbonyl groups and free sulfhydryl groups increased first and then decreased with increasing treatment time, while the opposite trend was found for the content of disulfide bonds. The solubility, emulsifying properties and foaming properties were significantly improved by jet cavitation, and this effect was more obvious at 5 g/100 mL than at 2 g/100 mL. These results showed that jet cavitation can change the structure of soybean 11S globulin, and consequently improve its solubility, foaming properties and emulsifying properties, resulting in more obvious unfolding and aggregation of soybean 11S globulin at 5 g/100 mL as well as more improved functional properties.