• 营养卫生 •

### 丹酚酸B对小鼠体内抗氧化和肠道微生物群落的影响

1. （1.河北科技大学生命科学与工程学院，河北 石家庄 050018；2.河北禾盛源生态农业科技开发有限公司，河北 唐山 063000）
• 出版日期:2020-05-15 发布日期:2020-05-15
• 基金资助:
河北省重点研发计划资助项目（18227135D）

### Effect of Salvianolic Acid B on Antioxidant Capacity and Intestinal Microflora in Mice

ZHAO Menghao, FENG Yinong, YIN Yuwen, LI Chengqian, SUN Guojie

1. (1. College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang 050018, China; 2. Hebei Heshengyuan Ecological Agriculture Technology Development Co. Ltd., Tangshan 063000, China)
• Online:2020-05-15 Published:2020-05-15

Abstract: The purpose of this study was to investigate the in vivo antioxidant properties of salvianolic acid B and its effect on the intestinal microbial community in mice. Kunming mice were administered by gavage with salvianolic acid B solution at low (30 mg/kg mb), medium (60 mg/kg mb), and high (120 mg/kg mb) doses for 42 successive days. At 24 h after the last administration, body mass was recorded, the organ coefficients of heart, liver, kidney and spleen were calculated, and the antioxidant indexes of blood and liver were detected. The results showed that the organ coefficients of liver in the medium-dose, high-dose and VC control groups were significantly lower than that in the normal group (P < 0.05). Similarly, the organ coefficient of kidney in the high-dose group was significantly lower than that in the normal (P < 0.05) and VC control groups (P < 0.01). The antioxidant indexes showed that compared with the normal group, MDA content was significantly decreased in the serum of mice in the middle-dose group (P < 0.05) as well as the high-dose and VC control groups (P < 0.01), and MDA content in the liver was also significantly decreased in the high-dose group (P < 0.05). In contrast, serum glutathione (GSH) levels in the high-dose and VC groups were significantly increased (P < 0.05). Through high-throughput sequencing analysis, the structure of intestinal flora in mice from each group was dominated by Bacteroides, Firmicutes, Proteobacteria, Verrucomicrobia and Actinobacteria. According to LEfSe analysis, the relative abundances of Bacteroides vulgatus and Parabacteroides distasonis were significantly increased in the three dose groups relative to the normal group (P < 0.05), decreasing with increasing dose. Therefore, salvianolic acid B can improve the antioxidant capacity and the intestinal environment of mice, increasing the number of beneficial bacteria. This finding provides new ideas for the treatment of cardiovascular diseases with salvianolic acid B.