食品科学 ›› 2020, Vol. 41 ›› Issue (14): 109-115.doi: 10.7506/spkx1002-6630-20190526-309

• 生物工程 • 上一篇    下一篇

应用高通量测序技术检测小麦储藏过程中真菌群落的变化

岳晓禹,张 华,陈威风,许文涛,郭明璋   

  1. (1.河南牧业经济学院食品工程学院,河南 郑州 450046;2.中国农业大学食品科学与营养工程学院,北京 100083)
  • 出版日期:2020-07-25 发布日期:2020-07-29
  • 基金资助:
    国家自然科学基金地方联合项目(U1404332);河南省科技创新人才支持计划项目(16HASTIT017)

Using High-throughput Sequencing to Study the Development of Fungal Community in Stored Wheat

YUE Xiaoyu, ZHANG Hua, CHEN Weifeng, XU Wentao, GUO Mingzhang   

  1. (1. College of Food Engineering, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China; 2. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China)
  • Online:2020-07-25 Published:2020-07-29

摘要: 通过高通量测序技术研究仓储小麦中真菌群落的变化规律及影响因素,为小麦仓储条件优化提供参考。结果表明,仓储小麦中真菌污染以链格孢霉、曲霉、枝孢霉为主,其中曲霉属测得11 个种,是物种多样性最高的属。结果表明,小麦储藏时间可能对其中真菌群落有较大的影响,储存1、2、3 a的小麦真菌群落有各自的特征真菌。小麦在仓库中的储藏方位可能与其中的真菌群落关系较小。储存深度对小麦真菌群落有影响,其中粪壳菌纲的微生物随着储藏深度的增加而丰度变低。河南和黑龙江两地的仓储小麦真菌群落差异较大。本研究可以为仓储小麦中真菌群落的发生演变模型的建立提供基础数据支持,为仓储小麦真菌控制提供可行方案。

关键词: 小麦, 真菌群落, 高通量测序

Abstract: In this paper, high-throughput sequencing technology was used to study the development of the fungal community in stored wheat and the factors influencing it, aiming to provide useful information for optimizing the storage conditions of wheat. The results showed that Alternaria, Aspergillus, and Cladosporium were the most abundant fungal contaminants in stored wheat. Aspergillus was the genus with the highest diversity at the species level, including 11 species. Storage period of wheat had a great influence on the fungal community. The fungal communities in wheat stored for one, two and three years had their own characteristic fungi. The location of wheat in the warehouse had little relationship with the fungal community, while the depth of the warehouse affected the fungal community, and the abundance of Sordariomycetes decreased with the increase in storage depth. The fungal communities of stored wheat in different provinces were quite different. This study can provide basic data for the establishment of a model for describing the development of the fungal community in stored wheat, and also provide feasible schemes for fungal control in stored wheat in the future.

Key words: wheat, fungal community, high throughput sequencing

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