食品科学 ›› 2020, Vol. 41 ›› Issue (13): 148-159.doi: 10.7506/spkx1002-6630-20190528-339

• 营养卫生 • 上一篇    下一篇

纳豆枯草芽孢杆菌LNUB236所产纳豆激酶的溶栓活性

朱俊丰,刘帅,赵鹏燕,赵健,李佳增,王天琦,王丽,刘宏生   

  1. (1.辽宁大学生命科学院,辽宁 沈阳 110036;2.辽宁大学药学院,辽宁 沈阳 110036)
  • 出版日期:2020-07-15 发布日期:2020-07-29
  • 基金资助:
    沈阳市科技创新“双百工程”百项重大科技成果转化项目(Z17-5-078); 辽宁省科技厅大型仪器设备共享服务平台能力建设基金项目(kjhx2017028); 辽宁省高等学校国(境)外培养项目(2018LNGXGJWPY-YB006);沈阳市科技局科技成果转化推进计划项目(17-65-7-00); 辽宁省教育厅青年项目(LQN201711);辽宁省重点研发计划项目(2019JH2/10300041)

Thrombolytic Activity of Nattokinase Produced by Bacillus subtilis natto LNUB236

ZHU Junfeng, LIU Shuai, ZHAO Pengyan, ZHAO Jian, LI Jiazeng, WANG Tianqi, WANG Li, LIU Hongsheng   

  1. (1. School of Life Sciences, Liaoning University, Shenyang 110036, China; 2. School of Pharmacy, Liaoning University, Shenyang 110036, China)
  • Online:2020-07-15 Published:2020-07-29

摘要: 目的:通过体内、体外实验研究纳豆枯草芽孢杆菌LNUB236所产纳豆激酶溶栓活性。方法:利用血凝块溶解实验测定纳豆激酶体外溶栓活性;利用角叉菜胶诱导构建大鼠尾部血栓模型、氯化铁诱导构建颈动脉血栓模型和小鼠尾部出血时间实验测定纳豆激酶体内溶栓活性;利用急、慢性毒性实验测定纳豆激酶的安全性。结果:纳豆激酶明显提高了血凝块的溶解速率。同时,纳豆激酶显著缩短角叉菜胶诱导的大鼠尾部血栓长度,显著降低氯化铁诱导的颈动脉血栓模型的血栓质量(P<0.05),并能明显延长小鼠尾部的出血时间。在急、慢性毒性实验中,纳豆激酶组大鼠的组织无病理损伤,血液生化指标均处于正常范围内。结论:枯草芽孢杆菌LNUB236所产的纳豆激酶在体内外均有溶栓活性,且对机体无损害和毒性。本研究为今后利用纳豆枯草芽孢杆菌LNUB236发酵生产纳豆激酶制作功能食品提供了参考。

关键词: 纳豆激酶, 溶栓活性, 毒性

Abstract: Objective: The aim of this study is to evaluate the thrombolytic effects of nattokinase (NK) produced by Bacillus subtilis natto LNUB236 in vitro and in vivo. Methods: The thrombolytic effect of NK in vitro was determined by the dissolution rate of blood clots. Meanwhile, the in vivo effect was assessed by using a carrageenan-induced rat tail thrombosis model and a ferric chloride-induced carotid arterial thrombosis model as well as measuring mouse tail bleeding time. The toxicity of NK was measured by acute toxicity test (14 days) and chronic toxicity test (28 and 90 days) in rats. Results: NK significantly increased the dissolution rate of blood clots, while it significantly reduced the length of carrageenan-induced rat tail thrombus and the mass of ferric chloride-induced-carotid thrombus (P < 0.05). In addition, NK significantly prolonged the tail bleeding time of mice. In the acute and chronic toxicity tests, the tissues of the mice in the NK group had no pathological damage, and blood biochemical indexes were in the normal range. Conclusion: The NK produced by Bacillus subtilis natto LNUB236 exerts a thrombolytic activity both in vitro and in vivo, without any toxicity for the body. This study can lay the foundation for the development of functional foods containing NK produced by Bacillus subtilis natto LNUB236.

Key words: nattokinase, thrombolytic activity, toxicity

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