食品科学 ›› 2020, Vol. 41 ›› Issue (13): 249-259.doi: 10.7506/spkx1002-6630-20190528-348

• 专题论述 • 上一篇    下一篇

色谱及色谱-质谱技术在复杂基质中赤霉素痕量分析中的应用

姜长岭,代金霞,刘新,鲁成银,陈红平   

  1. (1.中国农业科学院茶叶研究所,浙江 杭州 310008;2.中国农业科学院研究生院,北京 100081;3.农业农村部茶叶产品质量安全风险评估实验室,浙江 杭州 310008)
  • 出版日期:2020-07-15 发布日期:2020-07-29
  • 基金资助:
    国家自然科学基金面上项目(31671941); 中国农业科学院创新团队茶叶质量与风险评估团队项目(CAAS-ASTIP-2014-TRICAAS-06); 现代农业产业技术体系建设专项(nycytx-19)

Chromatography Alone and Coupled with Mass Spectrometry for the Determination of Trace Gibberellins in Complex Matrices: A Review

JIANG Changling, DAI Jinxia, LIU Xin, LU Chengyin, CHEN Hongping   

  1. (1. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China; 2. Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3. Laboratory of Quality and Safety Risk Assessment for Tea Products, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China)
  • Online:2020-07-15 Published:2020-07-29

摘要: 随着微生物代谢工程技术在合成生物学等领域的广泛应用,该技术已被用于提取多种植物天然产物。花色苷是一种水溶性天然色素,能使花和果实着色,已被广泛应用于食品和化妆品行业。目前,花色苷主要从植物材料中提取,但是提取的花色苷具有纯度低和性质不稳定等缺点,因此利用微生物代谢工程技术替代传统方法提取花色苷具有重要意义。本文阐述了工程菌合成花色苷的研究进展,对酶的选择、运输调节、尿嘧啶核苷-5’-二磷酸葡萄糖供应量的调控以及工艺优化等方面进行综述。对花色苷合成代谢途径的了解将为实现微生物生产花色苷的产业化提供重要信息。

关键词: 花色苷, 微生物代谢, 酶工程, 代谢工程

Abstract: Gibberellins (GAs) are a class of plant hormones that are widely found in plants, which play a crucial role in plant growth, development, and response to environmental stress. GAs are a class of tetracyclic diterpenic acids whose basic structure is 20-carbon gibberellin. So far, 136 GAs have been found. As GAs have been extensively applied in agricultural products and intensive research efforts have been made on their toxicity in recent years, their potential to cause food safety issues has become a subject of growing concern. Accurate analysis of GAs are helpful for better understanding their metabolism, physiological activity and toxicity. Due to their instability and extremely low content (generally ng/g, even pg/g levels), the pretreatment techniques and analytical methods for the detection of GAs in complex matrices are being continuously updating. This article reviews the development and breakthroughs of pretreatment techniques and analytical methods for trace analysis of GAs over the past 10 years, and future development trends are discussed.

Key words: gibberellins, chromatography-mass spectrometry, trace analysis, pretreatment techniques, analytical methods

中图分类号: