食品科学 ›› 2020, Vol. 41 ›› Issue (13): 127-132.doi: 10.7506/spkx1002-6630-20190626-351

• 营养卫生 • 上一篇    下一篇

辣木源多肽组分改善D-半乳糖致衰小鼠氧化损伤作用

耿春阳,林恋竹,赵谋明,朱启源   

  1. (华南理工大学食品科学与工程学院,广东 广州 510641)
  • 出版日期:2020-07-15 发布日期:2020-07-29
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2017YFD0400200)

Peptide Fractions from Moringa oleifera Ameliorate D-Galactose-Induced Oxidative Damage in Mice

GENG Chunyang, LIN Lianzhu, ZHAO Mouming, ZHU Qiyuan   

  1. (School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China)
  • Online:2020-07-15 Published:2020-07-29

摘要: 目的:研究蓝莓花色苷的组成、抗氧化能力,探讨其对炎症、癌细胞增殖的抑制作用及可能的机制。方法:采用高效液相色谱-电喷雾二级质谱联用技术分析蓝莓提取物(blueberry extracts,BE)的花色苷组成;通过1,1-二苯基-2-三硝基苯肼自由基清除能力评价其抗氧化能力;测定BE对一氧化氮(nitrite oxide,NO)释放、炎症因子及相关蛋白表达的影响;测定BE对人结肠癌细胞的生长抑制作用,Western blot检测细胞周期、细胞凋亡相关蛋白。结果:BE中有14 种花色苷,有很强的抗氧化能力,能够显著抑制NO释放以及白细胞介素-6、肿瘤坏死因子-α的分泌,下调一氧化氮合酶、环氧合酶-2的蛋白表达,并且能够显著地抑制人结肠癌细胞的增殖、细胞周期蛋白-2的表达。结论:BE具有较强的抗炎活性并对人结肠癌细胞有很好的抑制作用。

关键词: 炎症性肠病, 蓝莓提取物, 花色苷组成, 抗氧化能力, 炎症, 结肠癌

Abstract: In order to evaluate the antioxidant activity of peptide fractions from Moringa oleifera, peptides from Moringa oleifera leaves (MOL-P) and seeds (MOS-P) were prepared by enzymatic hydrolysis followed by fractional ethanol precipitation. A D-galactose-induced aging mouse model was established to assess the effects of administration with MOL-P and MOS-P at low and high doses (200 and 500 mg/(kg mb·d) by gavaging for 8 weeks on body mass, liver and brain indices, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and malondialdehyde (MDA) levels in serum, liver and brain of aging mice. The results indicated that MOL-P and MOS-P could restore brain indices close to normal (P > 0.05). Compared with the model group, both of them could significantly improve GSH-Px activity in serum and SOD activity in liver (P < 0.05), and reduce MDA levels in serum, liver and brain. Low-dose MOL-P and high-dose MOS-P had the best effects on improving GSH-Px activity in serum (higher than that of the model group by 35.80% and 36.97%, respectively). MOS-P had the best effect on the improvement of liver SOD activity, at high and low doses increasing liver SOD activity by 26.08% and 34.20% compared with the model group, respectively. MOL-P and MOS-P both at high dose showed the strongest inhibitory effects on lipid peroxidation in brain tissue (MDA levels were lower than that of the model group by 41.28% and 44.95%, respectively). In addition, MOL-P was able to reduce MDA levels in serum and liver to the normal level (P > 0.05). Both MOL-P and MOS-P could effectively alleviate oxidative damage in liver caused by D-galactose as evaluated by hematoxylin-eosin (HE) staining. Therefore, the peptides from Moringa oleifera leaves and seeds can antagonize oxidative stress in vivo and have good anti-aging effects, making them potential functional ingredients in anti-aging foods.

Key words: Moringa oleifera leave, Moringa oleifera seeds, peptide, oxidative stress, anti-aging

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