食品科学 ›› 2020, Vol. 41 ›› Issue (1): 133-138.doi: 10.7506/spkx1002-6630-20190629-403

• 营养卫生 • 上一篇    下一篇

不同级分绿豆肽免疫活性的分析

刁静静,迟治平,刘妍兵,曲柳青,张丽萍   

  1. (1.黑龙江八一农垦大学 国家杂粮工程技术研究中心,黑龙江 大庆 163319;2.黑龙江八一农垦大学食品学院,黑龙江 大庆 163319)
  • 出版日期:2020-01-15 发布日期:2020-01-19
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2017YFD0401203;2018YFE0206300); 研究生创新科研项目(YJSCX2018-Y55)

Immunoregulatory Activity of Mung Bean Peptides

DIAO Jingjing, CHI Zhiping, LIU Yanbing, QU Liuqing, ZHANG Liping   

  1. (1. Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 2. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China)
  • Online:2020-01-15 Published:2020-01-19

摘要: 为了探明绿豆肽级分与其免疫活性的关系,本实验采用葡聚糖凝胶G-15层析柱对绿豆肽进行分级,并将得到的3 个级分绿豆肽进行巨噬细胞增殖率、糖原含量、核酸含量以及细胞因子等免疫活性指标测定,以此优化出具有较高免疫活性的绿豆肽,并对其氨基酸序列进行鉴定。结果表明,不同级分绿豆肽可促进巨噬细胞增殖,其中MBPH-3级分绿豆肽促进效果最佳,相比空白组增殖率提高9.05%。同时发现不同级分绿豆肽可促进糖代谢以及核酸含量的升高,上调巨噬细胞细胞因子(白细胞介素-6、白细胞介素-1β、肿瘤坏死因子-α)表达量,其中MBPH-3级分绿豆肽的白细胞介素-6、白细胞介素-1β、肿瘤坏死因子-α表达水平分别为1?566.47、593.32、828.13?pg/mL,相比空白组(136.55、139.61、436.18?pg/mL)差异显著(P<0.05);同时不同级分绿豆肽又可抑制脂多糖(lipopolysaccharide,LPS)诱导的巨噬细胞分泌细胞因子,肿瘤坏死因子-α、白细胞介素-1β、白细胞介素-6分泌量分别为2?152.94、1?058.81、859.96?pg/mL,相比LPS刺激组(2?923.19、3?707.09、2?880.74?pg/mL)具有明显的抑制效果。综上所述,MBPH-3绿豆肽免疫效果最佳,结构鉴定结果表明,MBPH-3的平均分子质量约903?Da,氨基酸序列为Asn-Asn-Tyr-Gly-Pro-Thr-Met。

关键词: 绿豆肽, 巨噬细胞, 细胞因子, 免疫调节

Abstract: In this study, we aimed to evaluate the immunoregulatory activity of mung bean peptides. Three peptides were separated from mung bean protein hydrolyzed with alkaline protease by Sephadex G-15 column chromatography, and they were screened for their immunoregulatory activity in terms of macrophage proliferation rate, glycogen content, nucleic acid content, and cytokine secretion. Among these peptides, peptide MBPH-3 had the highest immunoregulatory activity, which increased macrophage proliferation rate by 9.05% compared with the blank control group. At the same time, all three mung bean peptides could promote glucose metabolism, increase the content of nucleic acid, and enhance the expression of cytokines such as interleukin (IL), IL-1β and tumor necrosis factor-α (TNF-α). The expression levels of cytokines in the MBPH-3 treated group were 1 566.47, 593.32, 828.13 pg/mL respectively. These values were significantly higher than those in the blank control group (136.55, 139.61 and 436.18 pg/mL) (P < 0.05). Moreover, MBPH-3 could inhibit cytokine secretion induced by lipopolysaccharide (LPS) in macrophages, and the amounts of TNF-α, IL-1β and IL-6 secreted by MBPH-3 treated macrophages were 2 152.94, 1 058.81 and 859.96 pg/mL (compared to 2 923.19, 3 707.09 and 2 880.74 pg/mL for LPS stimulated macrophages), respectively. In summary, the immunoregulatory effect of MBPH-3 is the best among the three mung bean peptides. The average molecular mass of MBPH-3 is approximately 903 Da and its amino acid sequence is Asn-Asn-Tyr-Gly-Pro-Thr-Met.

Key words: mung bean peptide, macrophage, cytokine, immune regulation

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