食品科学 ›› 2020, Vol. 41 ›› Issue (13): 106-111.doi: 10.7506/spkx1002-6630-20190702-021

• 营养卫生 • 上一篇    下一篇

原花青素对铁超载大鼠肾脏铁含量、氧化应激及Fas、Bax基因表达的影响

云少君,褚东阳,何兴帅,张文芳,冯翠萍   

  1. (山西农业大学食品科学与工程学院,山西 太谷 030801)
  • 出版日期:2020-07-15 发布日期:2020-07-29
  • 基金资助:
    国家自然科学基金青年科学基金项目(31801551);山西省高等学校科技创新项目(2016150); 山西农业大学引进博士科研启动费项目(2013YJ30)

Effects of Grape Seed Proanthocyanidins on Renal Iron Content, Oxidative Stress and Fas and Bax Gene Expression in Rats with Iron Overload

YUN Shaojun, CHU Dongyang, HE Xingshuai, ZHANG Wenfang, FENG Cuiping   

  1. (College of Food Science and Engineering, Shanxi Agricultural University, Taigu 030801, China)
  • Online:2020-07-15 Published:2020-07-29

摘要: 目的:以铁含量、氧化应激状态、肌酐和尿素氮水平、病理切片以及Fas、Bax基因的表达量为观测点,探究葡萄籽原花青素(grape seed proanthocyanidins,GSPAs)对铁超载大鼠肾脏功能的营养干预作用。方法:40 只雄性SD大鼠随机分为对照组(NC组)、铁超载模型组(Model组)、原花青素组(GSPAs组)和实验组(Test组)。通过全自动血液分析仪检测红细胞(red blood cell,RBC)和血红蛋白(hemoglobin,Hb)水平;以电感耦合等离子体质谱仪测定肾脏组织中Fe含量;采用相应试剂盒测定血清总铁结合力(total iron binding capacity,TIBC)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力、超氧化物歧化酶(superoxide dismutase,SOD)活力、丙二醛(malondialdehyde,MDA)含量、总抗氧化力(total antioxidant activity,T-AOC)、肌酐(creatinine,CR)和尿素氮(urea nitrogen,BUN)水平;苏木精-伊红(hematoxylin-eosin,HE)染色检测肾脏组织切片;实时荧光定量聚合酶链式反应检测肾脏组织Fas与Bax基因的表达量。结果:相较于NC组,Model组大鼠RBC数目、Hb水平、肾脏中Fe含量、MDA含量、CR和BUN水平、Fas及Bax基因表达量显著升高(P<0.05或P<0.01),而TIBC、GSH-Px及SOD活力显著降低(P<0.05或P<0.01),T-AOC差异不显著(P>0.05)。GSPAs组Hb水平、T-AOC显著升高,Fas基因表达量显著下调(P<0.05或P<0.01)。与Model组相比,Test组RBC、Hb、MDA、CR、BUN水平及Fas、Bax基因表达量显著降低(P<0.05或P<0.01);TIBC、GSH-Px活力、SOD活力、T-AOC显著升高(P<0.05或P<0.01)。结论:GSPAs能够对铁过量引起的大鼠肾脏铁超载、氧化损伤、生理功能紊乱及细胞凋亡起到保护作用。

关键词: 葡萄籽原花青素, 铁负荷, 肾脏, 氧化应激, 凋亡

Abstract: Objective: The effects of grape seed proanthocyanidins (GSPAs) on renal function in iron-overload rats were investigated by observing the iron content, oxidative stress status, creatinine and urea nitrogen levels, pathological sections and expression of Fas and Bax genes. Methods: Forty male SD rats were randomly divided into normal control group (NC), iron overload model group (model, injected with 100 mg/kg mb of iron dextran every other day), GSPAs group (gavaged at 100 mg/kg mb on a daily basis) and GSPA + iron overload group. Red blood cell (RBC) count and hemoglobin (Hb) levels were measured by an automatic blood biochemical analyzer, and iron content in kidney tissue was determined by inductively coupled plasma mass spectrometry (ICP-MS). Serum total iron binding capacity (TIBC), glutathione peroxidase (GSH-Px) activity, superoxide dismutase (SOD) activity, malondialdehyde (MDA) concentration, total antioxidant capacity (T-AOC), creatinine (CR) and urea nitrogen (BUN) levels were measured by commercial kits. Kidney sections were examined histologically by hematoxylin-eosin (HE) staining. Reverse transcription real-time polymerase chain reaction was used to determine the expression of Fas and Bax genes in kidney tissues. Results: Compared with the NC group, the RBC count, Hb level, renal Fe content, MDA content, CR and BUN level, Fas and Bax gene expression in the model group were significantly increased (P < 0.05 or P < 0.01), while TIBC, GSH-Px activity and SOD activity were significantly decreased (P < 0.05 or P < 0.01). There was no significant difference in T-AOC between the two groups (P > 0.05). The Hb content and T-AOC in the GSPAs group were increased significantly, while the expression of the Fas gene was downregulated significantly (P < 0.05 or P < 0.01). Compared with the model group, the RBC number, Hb content, MDA content, CR and BUN levels, and Fas and Bax gene expression in the GSPA + iron overload group were significantly decreased (P < 0.05 or P < 0.01), while TIBC, GSH-Px activity, SOD activity and T-AOC were significantly increased (P < 0.05 or P < 0.01). Conclusion: Grape seed proanthocyanidins can protect kidney tissues from iron accumulation, oxidative damage, renal physiological dysfunction and apoptosis induced by iron overload in rats.

Key words: grape seed proanthocyandins, iron overload, kidney, oxidative stress, apoptosis

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