食品科学 ›› 2020, Vol. 41 ›› Issue (13): 169-173.doi: 10.7506/spkx1002-6630-20190702-030

• 营养卫生 • 上一篇    下一篇

甜玉米芯多糖对糖尿病大鼠的降血糖作用

马永强,张凯,王鑫,路雪纯,林枞雨   

  1. (1.哈尔滨商业大学食品工程学院,黑龙江 哈尔滨 150076;2.哈尔滨工业大学生命科学与技术学院,黑龙江 哈尔滨 150080)
  • 出版日期:2020-07-15 发布日期:2020-07-29
  • 基金资助:
    哈尔滨商业大学研究生创新基金项目(YJSCX2018-543HSD);黑龙江省哈尔滨商业大学校级科研项目(17XN022)

Hypoglycemic Effect of Sweet Corn Cob Polysaccharide on Diabetic Rats

MA Yongqiang, ZHANG Kai, WANG Xin, LU Xuechun, LIN Congyu   

  1. (1. College of Food Engineering, Harbin University of Commerce, Harbin 150076, China;2. School of Life Science and Technology, Harbin Institute of Technology, Harbin 150080, China)
  • Online:2020-07-15 Published:2020-07-29

摘要: 灵芝多糖(Ganoderma lucidum polysaccharide,GLP)是灵芝(Ganoderma lucidum)的重要活性物质之一,拥有优异的保健功效,具有较高的研究价值。本实验以菌种G055的GLP为研究对象,对其进行提取纯化并探究其对人皮肤成纤维细胞氧化应激损伤的防护机制。通过Sevag法脱蛋白,采用DEAE-52离子交换层析得到GLP I和GLP II两组分,通过正交试验确定GLP的提取纯化工艺,GLP的最优提取条件为料液比1∶35(m/V)、浸提温度65 ℃、浸提时间1.5 h,在该条件下进行验证,GLP提取率为(47.70±0.50)%,再现性良好;通过建立以H2O2诱导的人皮肤成纤维细胞氧化应激损伤模型,围绕细胞抗氧化酶体系以及Kelch-like ECH-associated protein-1(Keap1)-核因子-E2相关因子(nuclear factor-erythroid 2-related factor 2,Nrf2)/antioxidant response element(ARE)信号通路,探究GLP及其组分(GLP I和GLP II)在防护损伤方面的作用效果及机制。损伤前的保护处理和损伤后的修复处理下,GLP及其两组分均能够显著提高细胞的超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶水平(P<0.05),降低活性氧以及脂质过氧化产物丙二醛的水平;GLP激活了Keap1-Nrf2/ARE信号通路的关键调控因子Nrf2,抑制了负调控因子Keap1,进而促进了下游抗氧化酶基因(NQO1和HO-1)的表达。综上,GLP的防护作用与其抗氧化酶水平及Keap1-Nrf2/ARE信号通路密切相关。

关键词: 灵芝菌丝体多糖, 提取纯化, 防护, 氧化应激损伤, Keap1-Nrf2/ARE信号通路

Abstract: We have previously prepared a polysaccharide from sweet corn cobs named SCP-80-1 and have demonstrated it to have a good hypoglycemic effect in vitro. In this study, we evaluated the hypoglycemic effect of SCP-80-1 on streptozotocin (STZ)-induced diabetic rats. The diabetic animals were administered intragastrically with SCP at doses of 100, 200 and 400 mg/kg mb or metformin at 200 mg/kg mb for 28 days. The fasting blood glucose, glucose tolerance, insulin content, blood lipids, visceral organ indices and pancreatic tissue sections were measured. The results showed that SCP-80-1 could decrease the fasting blood glucose level and postmeal blood glucose level, and improve the glucose tolerance of diabetic rats. It was also found that SCP-80-1 could improve blood lipid levels by reducing the levels of total cholesterol, triglyceride, and low-density lipoprotein cholesterol and increasing the level of high-density lipoprotein cholesterol. In addition, SCP-80-1 could reduce organ swelling to a certain extent, repair the damaged islet tissue, and increase the number of islet B cells. Therefore, SCP-80-1 can effectively regulate the blood glucose level of diabetic rats, improve glucose tolerance and repair the injured islet tissue, indicating that this polysaccharide is a natural hypoglycemic agent that is promising for application in the treatment of diabetes mellitus.

Key words: diabetes mellitus, sweet corn cob polysaccharide, hypoglycemia, blood lipid level, pancreatic tissue

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