食品科学 ›› 2020, Vol. 41 ›› Issue (13): 120-126.doi: 10.7506/spkx1002-6630-20190703-053

• 营养卫生 • 上一篇    下一篇

原花青素对小鼠血脂代谢紊乱与肠道菌群干预的影响

杨昌铭,肖瀛,吴其国,尹志婷,周一鸣,周小理   

  1. (上海应用技术大学香料香精技术与工程学院,上海 201418)
  • 出版日期:2020-07-15 发布日期:2020-07-29
  • 基金资助:
    国家自然科学基金青年科学基金项目(31401504)

Preventive Effect of Proanthocyanidin on Gut Microbiome in Dyslipidemic Mice

YANG Changming, XIAO Ying, WU Qiguo, YIN Zhiting, ZHOU Yiming, ZHOU Xiaoli   

  1. (School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, China)
  • Online:2020-07-15 Published:2020-07-29

摘要: 为评价辣木源多肽组分在动物体内的抗氧化功效,本实验采用酶法、乙醇分级沉淀法制备辣木叶多肽组分(MOL-P)、辣木籽多肽组分(MOS-P),构建D-半乳糖致衰小鼠模型,考察MOL-P和MOS-P在低、高剂量(200、500 mg/(kg mb·d))下连续灌胃小鼠8 周,对衰老小鼠体质量,肝脏和脑的脏器系数,血清、肝脏和脑抗氧化酶活力和丙二醛(malondialdehyde,MDA)水平的影响。结果表明:MOL-P和MOS-P均能上调衰老小鼠脑系数至正常组水平(P>0.05);辣木源多肽组分能显著提高衰老小鼠血清谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力和肝脏超氧化物歧化酶(superoxide dismutase,SOD)活力(P<0.05),一定程度上降低血清、肝脏和脑组织MDA水平,其中低剂量MOL-P和高剂量MOS-P对血清GSH-Px活力的提升作用最好(分别比模型组提高了35.80%和36.97%);MOS-P对肝脏SOD活力提升作用最好,高、低剂量组分别比模型组提高了26.08%和34.20%;高剂量MOL-P和高剂量MOS-P对脑组织脂质过氧化的抑制作用最好(高剂量MOL-P和高剂量MOS-P脑组织MDA水平分别比模型组降低了41.28%和44.95%);MOL-P能将血清、肝脏MDA水平下调至正常组水平(P>0.05)。采用苏木精-伊红染色观察衰老小鼠肝脏组织损伤程度,结果表明,MOL-P和MOS-P均能有效缓解D-半乳糖所致肝脏氧化损伤。结论:辣木叶、辣木籽多肽组分均能在动物体内拮抗氧化应激,具有良好的抗衰老活性,可作为功能性因子用于抗衰老食品的研制。

关键词: 辣木叶, 辣木籽, 多肽, 氧化应激, 抗衰老

Abstract: The intervening effect of dietary supplementation of proanthocyanidin B2 on dysfunctional blood lipid metabolism was evaluated in a mouse model of hyperlipidemia induced by a high-fat diet, and the underlying mechanism was explored from the perspective of intestinal flora. C57BL/6 mice (5 weeks old) were randomly divided into three groups: control group (A), high-fat diet (HFD) group (B), and proanthocyanidin B2 treatment group (C). After feeding for 12 weeks, visceral organ indices, blood lipids, hepatic enzyme activities and antioxidant capacity in liver and colon tissues were determined, the intestinal flora was analyzed using Illumina high-throughput sequencing technology, and changes in short-chain fatty acid contents in the feces of the mice were detected by gas chromatography-mass spectrometry. The results showed that proanthocyanidin B2 significantly reduced the levels of serum total cholesterol, triglyceride and atherosclerosis index (P < 0.05), significantly increased lipoprotein lipase activity, hepatic lipase activity, total lipase activity, and antioxidant activity, and inhibited malondialdehyde accumulation as an indicator of oxidative damage (P < 0.05). Taxonomic classification of the mouse intestinal microbiome at the phylum and genus levels demonstrated that proanthocyanidin B2 could increase Bacteroidetes/Firmicutes ratio in the intestinal flora of the HFD-fed mice and result in significant differences in the abundance of 13 species of bacteria, including Escherichia-shigella, Bacteroidales_S24-7_group, Ruminococcaceae, Bifidobacterium and Parabacteroides. The dominant bacterial genera in the proanthocyanidin B2 group were Romboutsia, Lachnospiraceae and Parabacteroides. Proanthocyanidin B2 significantly increased the contents of butyric acid in the feces of the HFD-fed mice (P < 0.05). Therefore, proanthocyanidin B2 can regulate the intestinal flora structure, thus preventing high-fat?diet-induced?dyslipidemia.

Key words: proanthocyanidin B2, blood lipid metabolism, antioxidant, intestinal flora

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