食品科学 ›› 2020, Vol. 41 ›› Issue (12): 158-165.doi: 10.7506/spkx1002-6630-20190709-122

• 成分分析 • 上一篇    下一篇

基于UPLC-Q-TOF-MS分析酵母对葡萄酒中糖苷态香气物质释放的影响

安效辉,苗瀛心,陈倬,张欣珂,段长青,潘秋红   

  1. (中国农业大学食品科学与营养工程学院,葡萄与葡萄酒研究中心,农业农村部葡萄酒加工重点实验室,北京 100083)
  • 出版日期:2020-06-25 发布日期:2020-06-22
  • 基金资助:
    国家自然科学基金面上项目(31772278)

Effects of Yeasts on the Release of Glycosidically Bound Aroma Components from Wine Analyzed by Ultra-high Performance Liquid Chromatography-Quadrupole-Time-of-Flight-Mass Spectrometry

AN Xiaohui, MIAO Yingxin, CHEN Zhuo, ZHANG Xinke, DUAN Changqing, PAN Qiuhong   

  1. (Key Laboratory of Viticulture and Enology, Ministry of Agriculture and Rural Affairs, Center for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China)
  • Online:2020-06-25 Published:2020-06-22

摘要: 采用超高效液相色谱-四极杆-飞行时间-质谱法,研究2 种酵母Red Fruit和Zymaflore ST分别在‘北冰红’和‘威代尔’冰酒的发酵过程中,对不同糖苷态香气物质含量的影响,以及瓶储1 年后葡萄酒中糖苷态香气物质含量的变化。结果表明,‘威代尔’冰酒中有更丰富的糖苷态香气物质种类和含量。酵母Red Fruit和Zymaflore ST发酵的‘北冰红’冰酒中,戊糖基-葡萄糖苷类香气物质均略有下降,但酵母Zymaflore ST发酵对己糖基-葡萄糖苷类香气物质影响不显著。酵母Zymaflore ST发酵‘威代尔’冰酒中,戊糖基-葡萄糖苷、己糖基-葡萄糖苷和葡萄糖苷类的香气物质均显著降低,而酵母Red Fruit对糖苷类型的香气物质均无显著影响(己糖基-葡萄糖苷类除外)。瓶储1 年后,‘北冰红’冰酒中各种糖苷态香气物质含量均大幅降低。研究认为,酵母Red Fruit和Zymaflore ST对发酵过程中‘北冰红’糖苷态香气释放影响很小,糖苷态香气释放可能主要在陈酿阶段,而酵母Zymaflore ST发酵较有利于‘威代尔’糖苷态香气的释放。该结果为冰酒的酿造和陈酿工艺设计提供了参考。

关键词: 超高效液相色谱-四极杆-飞行时间质谱, 糖苷态香气物质, ‘北冰红’冰酒, ‘威代尔’冰酒

Abstract: This study investigated the effects of two kinds of yeasts, Red Fruit and Zymaflore ST, on the release of glycosidically bound aroma compounds during the fermentation of ‘Beibinghong’ and ‘Vidal’ ice wine, as well as the variation in these aroma compounds in fresh and bottled ‘Beibinghong’ ice wine after aging for one year, by means of ultra-high performance liquid chromatography-quadrupole-time-of-flight-mass spectrometry (UPLC-Q-TOF-MS). The results showed that ‘Vidal’ ice wine contained more abundant types and higher concentration of glycosidically-bound aroma compounds than did ‘Beibinghong’ ice wine. In ‘Beibinghong’ ice wine fermented with either yeast, the content of aroma pentosyl glucosides slightly decreased during fermentation, while Zymaflore ST had no significant impact on hexosyl glucosides. By contrast, in ‘Vidal’ ice wine fermented with Zymaflore ST, the contents of pentosyl glucosides, hexosyl glucosides and glucosides significantly dropped during fermentation, whereas Red Fruit appeared to have no effect on pentosyl glucosides or glucosides. After aging for one year, ‘Beibinghong’ ice wine showed a large decrease of the contents of glucosidically bound aroma compounds. Based on these results, it is considered that the effects of Red Fruit and Zymaflore ST on the release of glycosidically bound aroma compounds are limited during the fermentation of ‘Beibinghong’ ice wine, and these aroma precursors could be released mainly in the process of aging while Zymaflore ST fermentation was more favorable for the release of glycosidically bound aroma in ‘Vidal’ ice wine. The findings provide a reference for fermentation and aging process design of ice wine.

Key words: ultra-high performance liquid chromatography-quadrupole-time-of-flight-mass spectrometry, aroma precursors, ‘Beibinghong’ ice wine, ‘Vidal’ ice wine

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