食品科学 ›› 2020, Vol. 41 ›› Issue (18): 159-164.doi: 10.7506/spkx1002-6630-20190721-267

• 生物工程 • 上一篇    下一篇

甘肃河西走廊葡萄酒产区本土酒酒球菌发酵耐受性分析

祝霞,王璐璐,赵丹丹,王诗,韩舜愈,杨学山   

  1. (甘肃农业大学食品科学与工程学院,甘肃省葡萄与葡萄酒工程学重点实验室,甘肃?兰州 730070)
  • 出版日期:2020-09-25 发布日期:2020-09-18
  • 基金资助:
    甘肃省重点研发计划项目(17YF1NA060);甘肃省葡萄酒产业发展基金项目(201820-07;201820-08)

Analysis of Fermentation Tolerance of Native Oenococcus oeni in Hexi Corridor of Gansu Province

ZHU Xia, WANG Lulu, ZHAO Dandan, WANG Shi, HAN Shunyu, YANG Xueshan   

  1. (Gansu Key Lab of Viticulture and Enology, College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China)
  • Online:2020-09-25 Published:2020-09-18

摘要: 为筛选出具有良好发酵耐受性的本土酒酒球菌(Oenococcus oeni),以甘肃河西走廊葡萄酒产区分离鉴定的7?株O.?oeni为供试菌株,分别对其进行pH值、乙醇、SO2质量浓度和发酵温度等酿造因素的耐受性考察,选择耐受性较好的菌株进行复合因素(pH值×乙醇×SO2质量浓度)适应性分析。结果表明,7?株本土O.?oeni发酵耐受性存在差异,且同一菌株对不同因素的抗胁迫性也不相同;菌株MG-1、MG-7、QL-11、ZX-1在pH?3.2、乙醇体积分数14%及SO2质量浓度40?mg/L条件下均可生长增殖;影响菌株MG-1和MG-7生长的因素主次为乙醇体积分数>SO2质量浓度>pH值,影响菌株QL-11和ZX-1生长的因素主次为乙醇体积分数>pH值>SO2质量浓度。综合分析,供试菌株中ZX-1的发酵耐受性最强,其次为菌株MG-1、MG-7和QL-11,均具有一定的产业化应用潜力。

关键词: 酒酒球菌;发酵条件;耐受性

Abstract: In order to screen for native Oenococcus oeni with good fermentation tolerance, seven O. oeni strains isolated from the wine-producing area of the Hexi corridor of Gansu province were investigated for their tolerance to fermentation factors such as pH value, alcohol, SO2 concentration and fermentation temperature. Then, the strains with good fermentation tolerance were selected to analyze their adaptability to various combinations of pH value, alcohol content and SO2 concentration. The results showed that there were differences in fermentation tolerance among the seven strains, and that each strain had different resistance to different stress factors. Strains MG-1, MG-7, QL-11 and ZX-1 could grow and proliferate under the conditions of pH 3.2, alcohol content 14% and SO2 concentration 40 mg/L. In decreasing order of importance, the factors affecting the growth of strains MG-1, MG-7, QL-11 and ZX-1 were alcohol content, SO2 concentration and pH value, while those affecting the growth of strains QL-11and ZX-1 were alcohol content, pH value and SO2 concentration. Collectively, we demonstrate that strain ZX-1 has the strongest fermentation tolerance among the tested strains, followed by MG-1, MG-7 and QL-11, all of which have potential for industrial application.

Key words: Oenococcus oeni; fermentation conditions; tolerance

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