食品科学 ›› 1999, Vol. 20 ›› Issue (10): 16-18.

• 基础研究 • 上一篇    下一篇

平菇多糖的分离纯化及其对超氧自由基的效应

 杨海龙, 林燕文   

  1. 韩山师范学院化生系
  • 出版日期:1999-10-15 发布日期:2011-12-06

Separation and purification of pleurotus ostreatus pdysaccharide and its effect on O2

 YANG  Hai-Long, LIN  Yan-Wen   

  • Online:1999-10-15 Published:2011-12-06

摘要: 平菇经热水抽提,乙醇沉淀,Sevag法去蛋白,乙醇分级分离,再经DEAE-纤维素柱层析得纯化多糖PSI和PSII,分别经聚丙烯酰胺凝胶电泳分析都呈一条带。在体外产生超氧阴离子自由基O2的反应体系中,平菇多糖组分PSI和PSII在较低浓度下(<300mg/L)都有清除O2的作用,而在较高浓度下(>300mg/L)作用不明显。

关键词: 平菇多糖, 分离, 纯化, 效应

Abstract: Polysaccharide PSI and PSII were isolated from the fruit bodies of Pleurotus ostreatus and subsequently purified by ethanol fractionation and DEAE-Cellulose chromatography;their homogineity were analysed by polyacrylamide gel eletrophoresis.The effects of PSI and PSII eliminating O2 were also studied it indicated:PSI and PSII could eliminate O2 in low concentration (<300mg/L)but their effects of eliminating O2 were indistinctive in high concentration(>300mg/L).

Key words: leurotus ostreatus polysaccharide, isolate, purify, effect