食品科学 ›› 2001, Vol. 22 ›› Issue (2): 50-52.

• 工艺技术 • 上一篇    下一篇

多管藻(P.urceolata)复合氨基酸营养液的研制

 魏玉西, 张立新, 李钐, 杭瑚, 薛章芳, 于希敏   

  1. 青岛大学理工学院; 山东省莒南县人民医院; 青岛海丰集团新产品开发部
  • 出版日期:2001-02-15 发布日期:2012-02-15

Study on Preparation of Nutritive Liquid of Composite Amino Acids from Enzymatic Hydrolysis of P.urceolata

 WEI  Yu-Xi, ZHANG  Li-Xin, LI  Shan, HANG  Hu, XUE  Zhang-Fang, YU  Xi-Min   

  • Online:2001-02-15 Published:2012-02-15

摘要: 本研究就多管藻(Polysiphonia urceolata)的脱色方法及酶解工艺条件等进行了探讨。结果表明原料脱色用KMnO4效果较好,酶解的最佳工艺条件是使用中性蛋白酶与胰蛋白酶联合酶解法,总加酶量为5‰(w/w)在45℃水解4h后,再加0.2%NaOH碱解2h,蛋白质水解度和氨基酸转化率分别高达80.4%和39.6%。

关键词: 多管藻, 脱色, 酶解, 水解度, 复合氨基酸营养液

Abstract: This paper presented the results of decolouring and enzymatic hydrolysis technology of P.urceolata to prepare a nutritive liquid of composite amino acids.The results showed that KMnO4 was a good decolourant for P.urceolata.The optimum hydrolysis conditions were :using combined enzymes (AS1.389 protease and trypsin,5‰ totally) at 45℃ for 4 hours and then treating with 0.2% NaOH for 2 hours.The degree of protein hydrolysis was up to 80.4% ,and the transformation ratio of amino acids was 39.6%.