食品科学 ›› 2002, Vol. 23 ›› Issue (11): 59-62.

• 工艺技术 • 上一篇    下一篇

黑曲霉(As.n.XD-1)β-葡萄糖苷酶产酶条件研究

 杨胜远   

  1. 广西大学食品发酵工程研究所
  • 出版日期:2002-11-15 发布日期:2011-12-31

Study on β - glucodydase Production Conditions of Aspergillus niger(As. n. XD - 1)

 YANG  Sheng-Yuan   

  • Online:2002-11-15 Published:2011-12-31

摘要: 通过单因子试验和正交表试验,对黑曲霉(As.n.XD-1)β-葡萄糖苷酶(EC.3.2.1.21)产酶条件进行了研究。试验表明:黑曲霉(As.n.XD-1)产β-葡萄糖苷酶适宜于固体发酵,适宜条件为甘蔗渣与麦麸配比为2:3,酵母膏0.8%,加水比为1:3,初始pH值自然,于28℃发酵4d。在这个优化条件下酶活力达23.86U/ml。

关键词: 黑曲霉, &beta, -葡萄糖苷酶, 产酶条件

Abstract: β - glucosidase (EC. 3.2. 1.21) production conditions of Aspergillus niger (As. n. XD - 1) were investigated through single factor experimentation and orthogonal test. The results showed that solid state fermentation was the suitable way of β - glucosidase production were 2 portions of bagasse, 3 portions of wheat bran, 0. 8% yeast extract, 3 times of water, initial natural pH value, and fermentation at 28() for 4d. Under these conditions, the activity of β - glucosidase could reached 23. 86U/ml.

Key words: Aspergillus niger , β- glucosidase , Produced condition