食品科学 ›› 2002, Vol. 23 ›› Issue (12): 70-74.

• 工艺技术 • 上一篇    下一篇

银杏外种皮总黄酮提取工艺研究

 李春美, 何静仁, 杜红祥, 谢笔钧   

  1. 华中农业大学食品科技系
  • 出版日期:2002-12-15 发布日期:2011-12-31

Technology Study on Leaching Flavanoids from Ginkgo Extocarp

 LI  Chun-Mei, HE  Jing-Ren, DU  Hong-Xiang, XIE  Bi-Jun   

  • Online:2002-12-15 Published:2011-12-31

摘要: 本文考察了不同浓度的酒精,不同的浸提温度、料液比、浸提时间以及浸提次数对银杏外种皮总黄酮浸出的影响,并将所得粗提物用ADS-17大孔树脂进行初步分离纯化,结果表明银杏外种皮最佳浸提工艺为:用60%乙醇,料液比1∶12,温度为60℃,浸提两次(每次2~3h)。采用ADS-17大孔树脂,用70%洗脱,可将银杏外种皮黄酮进行初步纯化,纯化后提取物中总黄酮含量可达22.3%。银杏外种皮提取物在FeSO4-H2O2体系中有很强的清除•OH作用,其IC50值为0.18mg/ml。

关键词: 银杏外种皮, 总黄酮, 提取工艺, &bull, OH

Abstract: The method of leaching flavanoids from Ginkgo ectocarp was studied.The results showed that the optimal leaching conditions were:extracting agent:60% ethanol;temperature:60℃;MTS ratio:1∶12;and estracting time:2~3h for 2times.The crude flavonoids could be prelimilarily purified by the ADS -17 macroporous resin and it had strong scarenging action to•OH generated by FeSO4-H2O2 system.The IC50 value was 0 08mg/ml.

Key words: Ginkgo, Extocarp, Flavonoid, Leaching •OH