食品科学 ›› 2002, Vol. 23 ›› Issue (12): 94-97.

• 分析检测 • 上一篇    下一篇

不同生长季节银杏叶中有毒成分银杏酸含量的测定

 吴向阳, 仰榴青, 陈钧   

  1. 江苏大学化学化工学院,江苏大学药学院,江苏大学生物与环境工程学院
  • 出版日期:2002-12-15 发布日期:2011-12-31

Determination of Toxic Components-Ginkgolic Acids in Leaves of Ginkgo Biloba Collected within Different Vegetative Periods

 WU  Xiang-Yang, YANG  Liu-Qing, CHEN  Jun   

  • Online:2002-12-15 Published:2011-12-31

摘要: 建立了紫外分光光度法测定银杏叶中有毒成分银杏酸含量的方法,测定了不同生长季节银杏叶中银杏酸含量,并与HPLC法测定结果作了比较。银杏叶的正己烷提取物经一步简单的预净化处理后可在242nm和310nm波长进行测定,线性回归方程分别为:A=1.55×10-4+16.36×C(R=0.999)和A=0.031+9.08×C(R=0.997),两者的线性范围均为5.72~114.4mg/L,平均回收率分别为97.6%和97.5%,测定6次的RSD分别为2.1%和2.4%,两个波长处的测定结果都与HPLC法较为接近。实验表明,银杏叶中银杏酸的含量与生长季节有关,9月为适宜采收期。该法简便、快速、准确,线性范围宽,最低可测至5.72×10-6,可用于银杏叶和银杏茶中有毒成分银杏酸的定量分析。

关键词: 银杏叶, 银杏茶, 银杏酸紫外分光光度法, 高效液相色谱法

Abstract: The method for determination of toxic components -ginkgolic acids in leaves of Ginkgo biloba by Ultraviolet spectrophotometry was established. The contents of ginkgolic acids in leaves of Ginkgo biloba collected within different vegetative periods were determined by UV and HPLC. The leaves of Ginkgo biloba were extracted by n-hexane in Soxhlet apparatus, then concentrated under vacuum. The ginkgolic acids could be determined directly by UV spectrophotometry at 242 nm and 310 nm after one-pre-purified-step by silica gel column chromatography. The linear regressive equations were A=1.55×10-4+16.36×C(R=0.999) and A=0.031+9.08×C(R=0.997) respectively. Their linear scope was 5.72~114.4 mg/L. The average recovery of ginkgolic acids was 97.6% and 97.5%,RSD was 2.1% and 2.4%(n=6) respectively. The results being detected at both wavelength were close to that by HPLC. The results showed that the content of ginkgolic acids in the leaves of Ginkgo biloba is relative to the vegetative periods ,and leaves of Ginkgo biloba are best collected in September. Not only is the method found to be simple ,fast and accurate,but has wide linear scope. The limit of detection can be up to 5.72×10-6. And it can be used for determination of toxic components-ginkgolic acids in the leaves and teas of Ginkgo biloba.

Key words: Leaves of gingko biloba , Teas of gingko biloba , Gingkolic acids , Ultraviolet spectrophotometry HPLC