食品科学 ›› 2003, Vol. 24 ›› Issue (1): 55-61.

• 基础研究 • 上一篇    下一篇

不饱和脂影响破囊壶菌产DHA的研究

 吴克刚, 柴向华, 杨连生   

  1. 汕头大学海洋生物研究所,汕头市农业科学研究所,华南理工大学食品学院
  • 出版日期:2003-01-15 发布日期:2011-12-13

Study on Effect of Unsaturated Lipids on DHA Production by Thraustochytrium Roseum Fermentation

 WU  Ke-Gang, CHAI  Xiang-Hua, YANG  Lian-Sheng   

  • Online:2003-01-15 Published:2011-12-13

摘要: DHA是大脑及视力正常发育所必需的脂肪酸,也是预防心血管系统疾病的功能活性成分。近年来微生物发酵生产DHA的研究已成为热点,特别是破囊壶菌Thraustochytriumroseum产DHA被认为极具工业化生产的潜力。本文研究了不饱和脂对破囊壶菌T.roseumMF2产DHA的影响作用。实验结果表明T.roseumMF2能不同程度利用不饱和脂为碳源进行生长和产DHA,其中以紫苏油的DHA产量最高,浓度为20g/L时DHA产量为382mg/L;当以40g/L葡萄糖为主要碳源,培养基添加红花油或紫苏油能提高DHA的含量和产量,二者添加量为8g/L时DHA产量分别提高18.5%和28.5%。

关键词: 不饱和脂, 破囊壶菌, DHA

Abstract: DHA is a kind of necessary fatty a cid asa functionel compoent for brain an d eye developnent cardio-vascular diseases prevention Recen tly the research on DHA production by microorgacism fermentation became Especially Thraustochytrium was reckoned as potential microorganismto produce DHAindustrially.In this paper.the effect of unsaturated lip ide on DHAproduction by T.roseum MF2was studied.Results showed that T roseum MF2could utilize unsaturated lipid s in varying degrees for growth and pr oduction of DHA .When the concentration of perillaseed oil in the mediumwas 20g /L,DHAyield was 382mg /Lwhich was the hi ghest yield amongall.ItcouldenhanceDHAconte ntinlipidandDHAyieldwhensafflow eroilorperillaseedoilwas addedin to the meliumcontaining 40g /l goucose as main carbon source.DHAyield was r espectively raised by 18.5%and28.5%when either oils was added into mediumby 8g /L

Key words: uinsaturated lipids, Thraustochytriumroseum DHA