食品科学 ›› 2003, Vol. 24 ›› Issue (2): 105-106.

• 分析检测 • 上一篇    下一篇

RP-HPLC法测定参类保健酒中人参皂甙Rg_l、Re、Rb_l的含量

 万春花, 龙洲雄, 朱筱玲, 江海亮, 罗香   

  1. 江西省分析测试研究所
  • 出版日期:2003-02-15 发布日期:2011-12-13

Study on Assaying Ginsenosides in Wine by RP-HPLC

 WAN  Chun-Hua, LONG  Zhou-Xiong, ZHU  Xiao-Ling, JIANG  Hai-Liang, LUO  Xiang   

  • Online:2003-02-15 Published:2011-12-13

摘要: 采用反相液相色谱法同时测定参类保健酒中三种人参皂甙的含量,使用SymmetryCl8柱,乙腈-0.2%磷酸溶液为流动相,流速1.0ml/min,梯度洗脱,柱温35℃,波长203nm,本法快速准确、分离效果好、回收率大于95.3%。

关键词: 人参皂甙, 梯度洗脱, 反相液相色谱

Abstract: This paper proposed a method for assaying three ginsenosides in wine by RP-HPLC.Rg1、Re and Rbl could be separated on symmetry C18 column.with gradient elution of CH3CN-0.2%H3PO4 at a flow-rate of 1.0ml/min and then assayed at UV 203nm ,with the colum temperature 35℃.This method was simple,rapid and accurate and had a very good separation, The recoveries were above 95%.

Key words: ginsenoside Rgl、Re、Rbl, gradient elution, RP-HPLC