食品科学 ›› 2007, Vol. 28 ›› Issue (3): 112-117.

• 工艺技术 • 上一篇    下一篇

辅酶Q10纳米脂质体配方与工艺优化研究

 夏书芹, 许时婴, 张晓鸣   

  1. 江南大学食品学院; 江南大学食品学院 江苏无锡214036; 江苏无锡214036;
  • 出版日期:2007-03-15 发布日期:2011-12-31

Study on Optimization of Formulation and Preparation of Coenzyme Q10 Nanoliposomes

 XIA  Shu-Qin, XU  Shi-Ying, ZHANG  Xiao-Ming   

  1. School of Food Science and Technology, Southern Yangtze University, Wuxi 214036, China
  • Online:2007-03-15 Published:2011-12-31

摘要: 采用乙醇注入-超声法制备辅酶Q10纳米脂质体,以包封率、保留率、平均粒径以及平均粒径的变化程度作为响应指标,应用正交试验法优选辅酶Q10纳米脂质体的配方和制备工艺。最佳配方为磷脂:胆固醇:吐温80:辅酶Q10=2.5:0.4:1.8:1.2(W/W),水相为0.01mol/L磷酸盐缓冲液(pH7.4);最佳制备工艺条件为乙醇用量1ml,搅拌时间10min,水化温度45℃,超声功率450W。以优化配方和工艺制得的脂质体形态均匀,粒径分布范围在20~300nm之间,平均粒径为68nm,包封率高于95%,4℃下贮存四个月,粒径分布无显著变化,平均粒径的变化程度小于10%,保留率高于90%。经优化得到的辅酶Q10纳米脂质体配方合理、工艺简便可行、包封率高、稳定性好。

关键词: 辅酶Q10, 纳米脂质体, 正交设计, 制备

Abstract: The optimal formulation and preparation techniques of coenzyme Q10 (CoQ10) nanoliposomes were investigated. The nanoliposomes were prepared with ethanol injection and sonication method. The encapsulation efficiency (EE), retention rate (RR), average particle size (D0.5) and average particle size change (△D0.5)of CoQ10 nanoliposomes were taken as criteria,and the optimization of the formulation and preparation techniques of CoQ10 nanoliposomes were achieved by orthogonal array design. The optimum formulation consisted of phospholipid/cholestero/Tween 80/CoQ10 (2.5:0.4:1.8:1.2, W/W) with phosphate buffer solution (pH7.4, 0.01 mol/L) as hydration media. The optimum preparation parameters are as follows: ethanol (1ml), hydration time (10min), hydration temperature (45℃) and sonication power (450W). The CoQ10 nanoliposomes employing the optimal formulation and preparation parameters have homogeneous shape with particle sizes ranging from 20nm to 300nm, and with D0.5 about 68nm. The EE of CoQ10 nanoliposomes is greater than 95% with RR higher than 90 % and △D0.5 lower than 10% after stored at 4℃ for three months. The optimized formulation of CoQ10 nanoliposomes is reasonable in prescription, practicable in techniques, high in encapsulation efficiency and good in stability.

Key words: coenzyme Q10, nanoliposomes, orthogonal array design, preparation