关键词: 杜仲叶多糖, 提取, 响应面分析

Abstract: In this paper,aiming at the extraction of Eucommia ulmoides leaves polysaccharide(EULP), test factors and levels were firstly selected by one factor tests. Next, on the basis of this and according to the Box-Benhnken center-united experimental design principles, the method of response surface analysis with 3 factors and 3 levels was adopted. Then, the factors influencing the technological condition were determined by means of regression analysis. Response surface and contour were finally graphed with the extraction rate of Eucommia ulmoides leaves polysaccharide as the response value. The results showed that the optim um conditions of Eucommia ulmoides leaves polysaccharide extraction are: the extraction temperature of 84 ℃, extraction time of 1.3 h, ratio of material to water of 1:14.3. On these conditions the extraction rate of Eucommia ulmoides leaves is up to l.7756% with one time extraction.

Key words: Eucommia ulmoides leaves polysaccharide, extraction, response surface analysis