食品科学 ›› 2007, Vol. 28 ›› Issue (8): 152-156.

• 工艺技术 • 上一篇    下一篇

零余子多酚类物质的提取工艺及测定方法比较

 李伟, 程超, 莫开菊, 汪兴平   

  1. 湖北民族学院生物科学与技术学院; 湖北民族学院生物科学与技术学院 湖北恩施445000; 湖北恩施445000; 湖北恩施445000湖北民族学院; 湖北省生物资源保护与利用重点实验室;
  • 出版日期:2007-08-15 发布日期:2011-10-24

Optimiazation of Extracting Technology of Yam Bean Polyphenols and Comparison of Different Determination Methods

 LI  Wei, CHENG  Chao, MO  Kai-Ju, WANG  Xing-Ping   

  1. 1.School of Biological Science and Technology, Hubei Institute for Nationalities, Enshi 445000, China;2.Hubei Key Laboratory of Biological Resource Conservation and Utilization, Hubei Institute for Nationalities, Enshi 445000, China
  • Online:2007-08-15 Published:2011-10-24

摘要: 本研究主要采用析因试验对零余子多酚类的最佳提取工艺进行了探讨,同时比较了多酚类物质的两种不同测定方法的差异。实验结果发现:采用FC法测定时,零余子多酚的最佳提取工艺为:乙醇浓度为75.3%,乙醇与盐酸比为98.3:1.7,提取温度为82.5℃,料液比1:30,此时多酚得率为11.1615mg/g。采用普鲁士蓝法检测时,最佳提取条件为:乙醇浓度为70%,醇酸比为97.6:2.4,提取温度为96℃,料液比1:30,此时零余子多酚的最高得率为4.4503mg/g。采用不同的测定方法,零余子多酚的最佳提取工艺略有不同,而且最高得率也不同。

关键词: 零余子, 多酚, 提取, 析因, 测定方法

Abstract: The optimal extracting technology of yam bean polyphenols was studied by the fractional factorial design, as well as two kinds of determination methods were compared. The results were as following: When the FC was used, the optimal extracting conditions were ethanol concentration 75.3%, ratio of the ethanol to HCl 98.3:1.7, temperature 82.5 ℃, and ratio of material to solvent 1:30. Under these conditions the highest extracting rate was 11.1615 mg/g. When the Prussian blue was used, the optimal extracting conditions were ethanol concentration 70%, ratio of ethanol to HCl 97.6:2.4, temperature 96 ℃, ratio of material to solvent 1:30. Under these conditions the highest extracting rate was 4.4503 mg/g.

Key words: yam bean polyphenol, extraction, fractional factorial design, determination methods