食品科学 ›› 2007, Vol. 28 ›› Issue (8): 160-164.

• 工艺技术 • 上一篇    下一篇

超声波提取翻白草中熊果酸工艺优化研究

 李胜华, 伍贤进, 郁建平, 王丽娜, 胡美忠   

  1. 怀化学院生物工程系; 贵州大学生化营养研究所; 贵州大学生化营养研究所 湖南怀化418008; 湖南怀化418008; 贵州贵阳550025;
  • 出版日期:2007-08-15 发布日期:2011-10-24

Study on Optimization of Extraction Process of Ursolic Acid from Potentilla discolor Bge with Ultrasonic

 LI  Sheng-Hua, WU  Xian-Jin, YU  Jian-Ping, WANG  Li-Na, HU  Mei-Zhong   

  1. 1.Department of Bioengineering, Huaihua College, Huaihua 418008, China; 2.Institute of Biochemistry and Nutrient, Guizhou University, Guizhou 550025, China
  • Online:2007-08-15 Published:2011-10-24

摘要: 目的:筛选翻白草中熊果酸的最佳提取工艺。方法:采用超声波提取、正交试验优化及高效液相色谱法测定所得翻白草中熊果酸的含量。结果:翻白草中熊果酸的提取最佳工艺是乙醇浓度为75%,固液比为1:60,提取时间为60min,超声波功率为610W,各因素的影响依次为提取时间>固液比>乙醇浓度和超声波功率。结论:最佳优选结果用于验证实验具有较好的重现性。

关键词: 翻白草, 熊果酸, 提取

Abstract: Objective: To screen optimum the extraction process of ursolic acid from Potentilla discolor Bge. Methods: ultrasonics extraction was used to extract. The optimum extraction process was confimed by the orthogonal test. The content of ursolic acid in Potentilla discolor Bge. was determined by HPLC. Results: The optimum conditions of ultrasonics extraction are as follow: ethanol concentration 75%, time 60 min, ratio of material to solution 1:60, and power of extraction 610 W. The effects of factors on yield decrease progressively in the following order: extracting time>ratio of material to solution>ethanol concentration>power of extraction. Conclusion: The best optimized process has a good repeatability confimatory experiment.

Key words: Potentilla discolor Bge., ursolic acid, extraction