食品科学 ›› 2007, Vol. 28 ›› Issue (8): 174-177.

• 工艺技术 • 上一篇    下一篇

龙须菜琼胶多糖的提取、纯化与性能表征

 薛志欣, 杨桂朋, 王广策   

  1. 青岛大学化工学院; 中国海洋大学海洋化学理论与工程技术教育部重点实验室; 中国科学院海洋研究所 山东青岛266071中国海洋大学海洋化学理论与工程技术教育部重点实验室; 山东青岛266003中国科学院海洋研究所; 山东青岛266071; 山东青岛266003;
  • 出版日期:2007-08-15 发布日期:2011-10-24

Isolation, Purification and Characterization of Agar Polysaccharide from Gracilaria Lemaneiformis

 XUE  Zhi-Xin, YANG  Gui-Peng, WANG  Guang-Ce   

  1. 1.College of Chemical Engineering, Qingdao University, Qingdao 266071, China;2.Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266003, China; 3.Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
  • Online:2007-08-15 Published:2011-10-24

摘要: 龙须菜(Gracilaria Lemaneiformis)的热水提取物,经DE-22和Sephadex G-200柱层析纯化,得到一种含微量硫酸基的琼胶多糖,此多糖经Q-Sepharose柱层析鉴定为单一组分。紫外光谱显示它不含蛋白质、多肽及核酸,红外光谱揭示它含3,6-内醚半乳糖特征吸收以及微量的硫酸基,热学性质表明在其0~700℃升温过程中存在两个失重过程,旋光分析表明随温度的升高,该多糖水溶液存在一个由有序构象(螺旋结构)向无序构象(无规线团)转变的过程。

关键词:  , 龙须菜, 多糖, 分离纯化, 性能表征

Abstract: The crude polysaccharide was extracted from Gracilaria Lemaneiformis with hot water and was then purified by DE-22 and Sephadex G-200 column chromatograph. The purified polysaccharide contained little sulfate. Its homogeneity was identified with Q-Sepharose column chromatography. The ultraviolet spectrum showed that there are no protein and nucleic acids in the purified polysaccharide. The infrared spectrum indicated that there are special absorption of 3,6-anhydro-galactose and very little sulfate absorbance peaks. There are two weight loss courses during the increasing temperature from 0 to 700 ℃, while there is a transition course from double helix structure to random coil with increasing temperature in the polysaccharide solution.

Key words: Gracilaria Lemaneiformis, polysaccharide, isolation and purification, characterization